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V1888 Sigma

Visualization of Receptors in situ: Applications of Radioligand Binding

  •  ISBN-10 0-8493-0042-8

  •  ISBN-13 978-0-8493-0042-4

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publication info   E. Moyse and S.M. Krantic, CRC Press, 2001, 208 pp., hard cover
mfr. no.   CRC Press

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General description

Radioligand binding in situ is the most sensitive method for detecting available receptors. But the success and reliability of this approach are determined by a number of technical requirements and constraints. This book combines theory and practice. It covers procedures such as radioligand and tissue preparations, in vitro assays, autoradiographic emulsion handling and image analysis. It also provides detailed protocols and uses parallel columns to convey practical comments and advise, often not encountered in original research papers.

Table of Contents

LIGHT MICROSCOPY. Ligand Labeling with Radioisotopes. Tissue Preparation for Light Microscopy. Radioligand Binding Assay on Frozen Tissue Sections. Film (Dry) Radioautography. Liquid Emulsion (Wet) Radioautography. Quantification of Radioautographic Labeling. ELECTRON MICROSCOPY. Choice of Radioligand. Tissue Preparation. Radioligand Binding Assay on Thick Tissue Slices. Embedding and Ultrathin Sectioning. Radioautography. Coloration and Contrasting of Ultrathin Sections. Analysis and Quantification of Radioautographic Labeling.

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