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V2258 Sigma

Monoclonal Anti-Vimentin antibody produced in mouse

clone LN-6, ascites fluid

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies against Proteins/Bioactives/Markers/Receptors for Stem Cell Biology, Antibodies for Alzheimer′s Disease, Antibodies for Cardiac Stem Cells,
species reactivity   feline, bovine, pig, rat, rabbit, human, mouse, sheep
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using human tissue sections
  immunoprecipitation: suitable
  indirect immunofluorescence: suitable using cultured cells
  western blot: suitable
clone   LN-6, monoclonal
antibody form   ascites fluid
isotype   IgM
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... VIM(7431)
mouse ... Vim(22352)
rat ... Vim(81818)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

human thymic nuclear extract

General description

Vimentin is a 58 kD class III intermediate filament present mainly in mesenchymal cells. It regulates cell shape, motility and endurance of mechanical stress in mesenchymal cells. Monoclonal anti-vimentin antibody can be used for the localization of vimentin by various immunochemical assays like immunoblotting, immunohistochemistry and immunoprecipitation. Frozen B5 fixed and formalin fixed, paraffin embedded tissue sections can be stained using anti-vimentin antibody. Mouse monoclonal anti-vimentin antibody reacts specifically with an epitope of the cytoskeletal protein vimentin (58kD). The product shows cross reactivity with vimentin from bovine, human, pig, sheep, cat, rat, mouse and rabbit.

Specificity

Recognizes an epitope of the cytoskeleton protein vimentin that is not detected in human lymphoid cells or derived malignancies. It is therefore useful in differentiating sarcomas from lymphomas.

Application

Monoclonal Anti-Vimentin antibody can be used in immunocytochemistry and immunohistochemical staining. It can also be used in immunofluorescence staining (diluted 1: 500) .

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Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  2
Protocols & Articles

Articles

Alzheimer's Disease

Alzheimer's disease (AD) is the most common cause of dementia in the elderly and is characterized by gradual loss of cognitive functions. Hallmark pathohistological findings of AD include widespread ...
Carolyn L. Crankshaw
BioFiles v7 n2, 2011, 4–8
Keywords: Alzheimer Disease, Gene expression, Genetic, Genetics, Inflammation, Metabolism, Phosphorylations

Improving Reproducibility: Best Practices for Antibodies

In the midst of beeping lab timers, presentations and grant deadlines, it is easy to take for granted the quality of lab reagents.
Keywords: Cancer, Capillary electrophoresis, Cell disruption, Centrifugation, Chromatography, Clinical, Degradations, Electrophoresis, Enzyme-linked immunosorbent assay, Gene expression, High performance liquid chromatography, Immunohistochemistry, Immunoprecipitation, Liquid chromatography mass spectrometry, Mass spectrometry, Mass spectrometry detection, Pharmaceutical, Purification, Sequencing, Titrations, Western blot

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Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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