Antibodies against Proteins/Bioactives/Marke|
|species reactivity||feline, bovine, pig, rat, rabbit, human, mouse, sheep|
|application(s)||immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using human tissue sections|
|indirect immunofluorescence: suitable using cultured cells|
|western blot: suitable|
|antibody form||ascites fluid|
|contains||15 mM sodium azide|
|shipped in||dry ice|
human ... VIM(7431)|
mouse ... Vim(22352)
rat ... Vim(81818)
human thymic nuclear extract
Vimentin is a 58 kD class III intermediate filament present mainly in mesenchymal cells. It regulates cell shape, motility and endurance of mechanical stress in mesenchymal cells4. Monoclonal anti-vimentin antibody can be used for the localization of vimentin by various immunochemical assays like immunoblotting, immunohistochemistry and immunoprecipitation. Frozen B5 fixed and formalin fixed, paraffin embedded tissue sections can be stained using anti-vimentin antibody. Mouse monoclonal anti-vimentin antibody reacts specifically with an epitope of the cytoskeletal protein vimentin (58kD). The product shows cross reactivity with vimentin from bovine, human, pig, sheep, cat, rat, mouse and rabbit.
Recognizes an epitope of the cytoskeleton protein vimentin that is not detected in human lymphoid cells or derived malignancies. It is therefore useful in differentiating sarcomas from lymphomas.
Monoclonal Anti-Vimentin antibody can be used in immunocytochemistry1 and immunohistochemical staining2. It can also be used in immunofluorescence staining (diluted 1: 500) 3.
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~1 mg/mL, clone V9, purified immunoglobulin, buffered aqueous solution
clone VIM-13.2, ascites fluid
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Alzheimer's disease (AD) is the most common cause of dementia in the elderly and is characterized by gradual loss of cognitive functions. Hallmark pathohistological findings of AD include widespread ...
Carolyn L. Crankshaw
BioFiles v7 n2, 2011, 4–8
Keywords: Alzheimer Disease, Gene expression, Genetic, Genetics, Inflammation, Metabolism, Phosphorylations
As a leading supplier of antibodies, Sigma® Life Science is committed to providing researchers the antibody they need with the quality they deserve. Our Antibody Explorer Search Tool provides easy ac...
Keywords: Buffers, Cell biology, Epigenetics, Immunohistochemistry, Immunoprecipitation, Molecular biology, Neuroscience, Western blot
2. Angiotensin-converting enzyme inhibition attenuates myonuclear addition in overloaded slow-twitch skeletal muscle. Christopher M. Westerkamp Am. J. Physiol. Regul. Integr. Comp. Physiol. 289(4), R1223-31, (2005)
LN-6: a monoclonal antibody to vimentin expressed in non-hematopoietic mesenchymal cells and derived tumors and reactive in B5-fixed, paraffin-embedded tissues. Stathopoulos, E., et al. J. Histochem. Cytochem. 37, 1363, (1989)
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