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V4505 Sigma

Monoclonal Anti-Vinculin antibody produced in mouse

clone VIN-11-5, ascites fluid

Synonym: Anti-VCL

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Actin-associated Proteins/Myosin, Antibodies to Cell and Organelle Proteins,
species reactivity   bovine, mouse, chicken, human
application(s)   indirect immunofluorescence: 1:50 using cultured chicken fibroblasts
  microarray: suitable
  western blot: suitable
clone   VIN-11-5, monoclonal
antibody form   ascites fluid
isotype   IgG1
mol wt   antigen mol wt 116 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... VCL(7414)
mouse ... Vcl(22330)
biological source   mouse
conjugate   unconjugated
Featured Industry   Research Pathology

Description

Immunogen

smooth muscle vinculin from chicken gizzard.

General description

The vinculin (VCL) gene containing 22 exons is mapped to human chromosome 10q22.1-q23. The gene codes for a 117kDa cytoskeletal protein present in adherens type cell junctions. VCL is a ubiquitously expressed cell-adhesion protein. The protein is characterized with a globular head and a tail domain, which are interconnected by a short proline-rich sequence, an intramolecular interaction between the head and tail conceal many ligand-binding sites in the protein.

Specificity

The antibody reacts best with cultured chicken fibroblasts. Labeling also may be obtained with bovine, human, or mouse cells.

Application

Monoclonal Anti-Vinculin antibody produced in mouse has been used in:
• western blotting.
• immunofluorescence.
• immunocytochemistry.

Biochem/physiol Actions

Vinculin (VCL) is a cytoskeletal protein, which plays a vital role in regulation of cell-to-cell and cell to matrix junctions. VCL facilitates normal embryonic development and cardiac function. Aberration or mutation of the gene leads to cardiomyopathy. VCL also plays a crucial role in cell migration and morphogenesis. VCL might facilitate the binding of integrins and cadherins to the actin cytoskeleton. Vinculin plays an important role in integrin αIIbβ3 signaling and function, which is required for platelet aggregation.

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Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  3
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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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V9264 Anti-Vinculin antibody, Mouse monoclonal, clone hVIN-1, purified from hybridoma cell culture
92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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