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V4888 Sigma

Anti-VSV-G antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-Vesicular Stomatitis Virus glycoprotein



Related Categories Alphabetical Index, Antibodies, Molecular Biology, Primary Antibodies, Proteomics,
biological source   rabbit
antibody form   affinity isolated antibody
clone   polyclonal
form   buffered aqueous solution
species reactivity   mammals
concentration   ~1.0 mg/mL
application(s)   immunoprecipitation: 0.5 μg using VSV-G tagged fusion proteins from transfected mammalian cell lysates
  indirect immunofluorescence: 1.0 μg/mL using VSV-G tagged fusion proteins in methanol/acetone fixed transiently transfected cells
  western blot: 0.1-0.2 μg/mL using VSV-G tagged fusion proteins from transfected mammalian cell lysates
conjugate   unconjugated
shipped in   dry ice
storage temp.   −20°C


General description

The Anti-VSV-G rabbit antibody reacts with amino- and carboxyl-terminal VSV-G tagged fusion proteins.


synthetic peptide corresponding to amino acids 497-511 (N-Tyr-Thr-Asp-Ile-Glu-Met-Asn-Arg-Leu-Gly-Lys-C) of the Vesicular Stomatitis Virus glycoprotein (VSV-G), conjugated to maleimide activated KLH via a cysteine residue added at the carboxyl terminus. The antibody recognizes the VSV-G tag at both the C-terminus and N-terminus.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide as a preservative.


Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)
Western Blotting (1 paper)
Western blotting following immunoprecipitation (1 paper)

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Safety Information

NONH for all modes of transport
WGK Germany 
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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

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