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V5255 Sigma

Monoclonal Anti-Vimentin antibody produced in mouse

clone VIM-13.2, ascites fluid

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies against Proteins/Bioactives/Markers/Receptors for Stem Cell Biology, Antibodies for Alzheimer′s Disease, Antibodies for Cardiac Stem Cells,
species reactivity   human, frog, rat, rabbit, mouse, chicken
application(s)   indirect immunofluorescence: 1:200 using cultured chicken fibroblasts
  western blot: 1:500 using human fibroblasts cell extract
clone   VIM-13.2, monoclonal
antibody form   ascites fluid
isotype   IgM
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... VIM(7431)
mouse ... Vim(22352)
rat ... Vim(81818)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

human foreskin fibroblasts

General description

Vimentin is a 53 kD type III intermediate filaments expressed in breast myoepithelial cells, osteocytes, langerhans cells of the skin, schwann cells and astrocytes. It has a crucial role in motility, maintenance of cell shape and endurance of mechanical stress of mesenchymal cells. Monoclonal anti-vimentin antibody can be used in immunofluorescent labeling of frozen tissue sections to localize vimentin in fibroblasts, endothelial cells, lymphoid tissues and melanocytes. It can also be used in western blotting. Mouse anti-vimentin antibody reacts specifically with vimentin. This product has also shown best reactivity with cultured chicken fibroblasts and can also react with human and mouse cells or tissues.

Application

Monoclonal anti-vimentin antibody can be used in immunocytochemical localization of vimentin in normal and pathological tissue of mesenchymal origin. It can also be used in immunoblotting and indirect immunofluorescence (1:200). Further, anti-vimentin antibody can be used to distinguish tumors and metastatic lesions derived from sarcomas, lymphomas, and melanomas.

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Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  3
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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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