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V5507 Sigma

Monoclonal Anti-VSV Glycoprotein antibody produced in mouse

clone P5D4, ascites fluid

Synonym: Monoclonal Anti-VSV Glycoprotein



Related Categories Alphabetical Index, Antibodies, Antibodies to Peptide and Protein Fusion Tags, Molecular Biology, Primary Antibodies,
biological source   mouse
antibody form   ascites fluid
clone   P5D4, monoclonal
contains   15 mM sodium azide
application(s)   immunocytochemistry: suitable
  immunoprecipitation: suitable
  western blot: 1:100,000 using whole cell extracts expressing VSV-G tagged fusion protein
isotype   IgG1
conjugate   unconjugated
shipped in   dry ice
storage temp.   −20°C



synthetic peptide containing the 15 carboxy-terminal amino acids (497-511) of Vesicular Stomatitis Virus Glycoprotein (VSV-G), conjugated to KLH.


The antibody recognizes an epitope containing the five carboxy-terminal amino acids of VSV Glycoprotein. In infected cells, the antibody localizes the immature forms of VSV-G in the rough endoplasmic reticulum (RER) and in the cisternae of Golgi complex, as well as mature VSV-G at the cell surface and in the budding virus. The antibody does not stain the secreted form of VSV-G which lacks the membrane and the cytoplasmic domain. This antibody has been used for studies on the role of the cytoplasmic domain on newly-synthesized VSV-G during transfer to the plasma membrane and cell surface, using micro-injected antibody, immunoblotting, immunoprecipitation, immunocytochemistry and immunoelectron microscopy. The antibody has been used for the detection, immunoprecipitation and immunocytochemical staining of exogenously introduced constructs tagged with the carboxyl-terminus of VSV-G. This tag does not interfere with the function of the studied protein and can be specifically recognized by the P5D4 antibody without cross-reaction with any endogenous protein.


Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Flow cytometry/Cell sorting (1 paper)

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NONH for all modes of transport
WGK Germany 


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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

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