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V6630 Sigma

Monoclonal Anti-Vimentin antibody produced in mouse

clone V9, ascites fluid

Synonym: Monoclonal Anti-Vimentin

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies against Proteins/Bioactives/Markers/Receptors for Stem Cell Biology, Antibodies for Alzheimer′s Disease, Antibodies for Cell Biology,
species reactivity   horse, monkey, bovine, rabbit, pig, canine, gerbil, rat, chicken, human, feline, hamster
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40 using human tissue
  western blot: 1:200 using human fibroblasts HS-68
clone   V9, monoclonal
antibody form   ascites fluid
isotype   IgG1
mol wt   antigen mol wt ~58 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... VIM(7431)
rat ... Vim(81818)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

pig eye lens vimentin.

General description

Vimentin is encoded by a single-copy gene mapped to human chromosome 10p13. 53kDa vimentin protein belongs to type III intermediate filament (IF) family and is specifically expressed in normal mesenchymal cells. Vimentin protein with 466 amino acids is characterized with a highly conserved a-helical “rod” domain that is flanked by non-α-helical amino- terminal “head” and carboxy-terminal “tail” domain.

Specificity

The antibody localizes vimentin in fibroblasts, endothelial cells, lymphoid tissue and melanocytes in immunohistochemical staining. It also stains vimentin in sarcomas, lymphomas, and melanomas.

Application

Monoclonal Anti-Vimentin antibody produced in mouse has been used as a primary antibody to localize vimentin by immunohistochemistry (IHC) and for immunoprecipitation of vimentin.

Monoclonal Anti-Vimentin antibody produced in mouse has been used:
• in immunofluorescence Analysis.
• in western blot.
• anti-vimentin antibody is injected into mature oocytes before embryo manipulations to bind endogenous vimentin (VIM).
• in immunocytochemistry.

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Biochem/physiol Actions

Vimentin plays a vital role in maintaining the structural integrity of the cells. Overexpression of vimentin is observed in prostate cancer, breast cancer, endometrial cancer, central nervous system (CNS) tumors, malignant melanoma and gastrointestinal tumors including pancreatic, colorectal and hepatic cancers. Thus, vimentin can act as a target for developing therapeutics for the treatment of cancer. Vimentin plays a crucial role in the vascular endothelial growth factor receptor-1 (VEGFR-1)-induced activation of the protein kinase G (PKG) 1 signaling pathway, stimulating regression of cardiomyocyte hypertrophy. Vimentin filaments are implicated in various physiological process including migration, maintenance of cell shape and tolerance of mechanical stress of mesenchymal cells. Vimentin interacts with LARP6 (La ribonucleoprotein domain family member 6) and stabilizes type I collagen mRNAs and might play a vital role in the development of tissue fibrosis. Vimentin plays an essential role in the maintenance of lens integrity, therefore, mutation of the vimentin gene causes dominant and pulverulent cataract.

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Safety Information

RIDADR  NONH for all modes of transport
Protocols & Articles

Articles

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Peer-Reviewed Papers
15

References

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C9080 Monoclonal Anti-Vimentin−Cy3 antibody produced in mouse, clone V9, purified immunoglobulin, buffered aqueous solution

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