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V6630 Sigma

Monoclonal Anti-Vimentin antibody produced in mouse

clone V9, ascites fluid

Synonym: Monoclonal Anti-Vimentin

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies against Proteins/Bioactives/Markers/Receptors for Stem Cell Biology, Antibodies for Alzheimer′s Disease, Antibodies for Cell Biology,
species reactivity   horse, monkey, bovine, rabbit, pig, canine, gerbil, rat, chicken, human, feline, hamster
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:40 using human tissue
  western blot: 1:200 using human fibroblasts HS-68
clone   V9, monoclonal
antibody form   ascites fluid
isotype   IgG1
mol wt   antigen mol wt ~58 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... VIM(7431)
rat ... Vim(81818)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

pig eye lens vimentin.

Specificity

The antibody localizes vimentin in fibroblasts, endothelial cells, lymphoid tissue and melanocytes in immunohistochemical staining. It also stains vimentin in sarcomas, lymphomas, and melanomas.

Application

Monoclonal Anti-Vimentin antibody produced in mouse has been used as a primary antibody to localize vimentin by immunohistochemistry (IHC) and for immunoprecipitation of vimentin.

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Biochem/physiol Actions

Vimentin is the major cytoskeletal component in immature glia. It has a molar mass of 57kDa. It is an intermediate filament protein and serves as a substrate for several caspases. The cleavage of this protein by caspase dismantles intermediate filaments and promotes apoptosis.

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