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V8012 Sigma

Monoclonal Anti-V5 antibody produced in mouse

~1 mg/mL, purified immunoglobulin, clone V5-10

Synonym: Monoclonal Anti-V5

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies and Conjugates, Antibodies to Peptide and Protein Fusion Tags, Molecular Biology,
biological source   mouse
antibody form   purified immunoglobulin
clone   V5-10, monoclonal
concentration   ~1 mg/mL
application(s)   immunocytochemistry: 1-2 μg/mL
  western blot: 0.5-1 μg/mL
conjugate   unconjugated
shipped in   dry ice
storage temp.   −20°C

Description

Application

Antibody is suitable for ELISA, immunoblotting and immunocytochemistry.

Specificity

Recognizes V5 Tag (GKPIPNPLLGLDST) fusion proteins

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

General description

The mouse mooclonal Anti-V5 antibody reacts specifically with the V5-taged recombinant fusion proteins expressed in transfected mammalian cells or produced by in vitro translation.

Price and Availability


Amplified Detection

Amicon Pro Purification System
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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