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V8012 Sigma

Monoclonal Anti-V5 antibody produced in mouse

~1 mg/mL, purified immunoglobulin, clone V5-10

Synonym: Monoclonal Anti-V5

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies and Conjugates, Antibodies to Peptide and Protein Fusion Tags, Molecular Biology,
biological source   mouse
antibody form   purified immunoglobulin
clone   V5-10, monoclonal
concentration   ~1 mg/mL
application(s)   immunocytochemistry: 1-2 μg/mL
  western blot: 0.5-1 μg/mL
conjugate   unconjugated
shipped in   dry ice
storage temp.   −20°C

Description

Specificity

Recognizes V5 Tag (GKPIPNPLLGLDST) fusion proteins

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Flow cytometry/Cell sorting (1 paper)
Western Blotting (2 papers)

General description

The mouse mooclonal Anti-V5 antibody reacts specifically with the V5-taged recombinant fusion proteins expressed in transfected mammalian cells or produced by in vitro translation.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

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Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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