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V8137 Sigma

Anti-V5 antibody produced in rabbit

IgG fraction of antiserum

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies and Conjugates, Antibodies to Peptide and Protein Fusion Tags, Molecular Biology,
biological source   rabbit
antibody form   IgG fraction of antiserum
clone   polyclonal
application(s)   immunocytochemistry: 5 μg/mL using V5 tagged fusion protein in methanol-acetone fixed transiently transfected 293T cells
  immunoprecipitation: 5 μg/mL using V5 tagged fusion protein from extracts of transfected cells
  western blot: 2.5 μg/mL using V5 tagged fusion protein expressed in whole extracts of transfected cells
conjugate   unconjugated
shipped in   dry ice
storage temp.   −20°C

Description

Specificity

Recognizes V5 Tag (GKPIPNPLLGLDST) fusion proteins

Physical form

Solution in 0.01M phosphate buffered saline, pH 7.4 containing 15 mM sodium azide.

Application

Anti-V5 antibody produced in rabbit has been used in:
• immunocytochemistry
• immunoprecipitation
• western blot

General description

V5 is an amino coterminal protein consisting of a C-terminus cysteine-rich region that resembles a cysteine-rich metal binding domain. It is encoded by the "P" gene of the paramyxovirus SV5.
The rabbit Anti-V5 synthetic peptide antibody reacts with V5 tagged recombinant fusion proteins expressed in transfected mammalian cells and from in vitro translation.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

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Peer-Reviewed Papers
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