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W0393 Sigma

Anti-WRN antibody, Mouse monoclonal

clone 195C, purified from hybridoma cell culture

Synonym: Anti-RECQ3, Anti-RECQL2, Anti-RECQL3, Anti-Werner syndrome helicase, Anti-Werner syndrome protein

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Stress, Antibodies to DNA Repair Proteins,
species reactivity   human
application(s)   immunohistochemistry: suitable
  immunoprecipitation: suitable
  indirect ELISA: suitable
  western blot: 2-4 μg/mL using nuclear cell extract of HeLa cells
clone   195C, monoclonal
concentration   ~1 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt ~167 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... WRN(7486)
biological source   mouse
conjugate   unconjugated

Description

Immunogen

recombinant fusion protein fragment of human WRN (amino acids 1074-1432).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

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Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
WGK Germany  2
Protocols & Articles

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Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

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W1642 Anti-WRN (C-terminal) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution

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