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W0890 Sigma

Anti-WASP antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous glycerol solution

Synonym: Anti-Wiskott-Aldrich Syndrome Protein

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Actin-associated Proteins/Myosin, Antibodies to Cell and Organelle Proteins,
species reactivity   mouse, human
application(s)   immunoprecipitation: 4 μg using 0.5 mg of human Jurkat and Raji and murine BAF-3 RIPA lysates
  western blot: 2 μg/mL using RIPA lysates from human Jurkat and Raji and murine BAF-3 cells
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous glycerol solution
mol wt   antigen mol wt 65 kDa
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... WAS(7454)
mouse ... Was(22376)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

GST fusion protein corresponding to residues 1-379 of human WASP

Antigen Background

WASP interacts with the GTPase CdC42 and seems to provide a link between Cdc42 and the actin cytoskeleton.

Physical form

Solution in 0.07 M Tris-glycine, pH 7.4, containing 0.105 M NaCl, 30% glycerol and 0.035% sodium azide

Specificity

WASP may migrate on SDS-PAGE at an apparent molecular weight ranging from 59-75 kDa. In some cell lines, an unknown band (85 kDa) is seen. Cross-reactivity with N-WASP, a homologue protein isolated from brain, has not been determined.

Preparation Note

Purified using protein A

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Safety Information

WGK Germany  1
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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
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