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X2252 Sigma

Xanthine Oxidase microbial

lyophilized powder, ≥7 units/mg solid

Synonym: XOD, Xanthine:oxygen oxidoreductase

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Properties

Related Categories 1.1.x.x Acting on hydroxyl groups, 1.x.x.x Oxidoreductases, Biochemicals and Reagents, Cell Biology, Enzyme Class Index,
form   lyophilized powder
mol wt   mol wt ~160 kDa
shipped in   wet ice
storage temp.   −20°C
Gene Information   Escherichia coli K12 ... xdhA(947116), xdhB(947205), xdhC(945148)

Description

Application

This enzyme is useful for enzymatic determination of inorganic phosphorus, 5′-nucleotidase and adenosine deaminase when coupled with Purine-nucleoside phosphorylase (PNP-301) and uricase (UAO-201, UAO-211).

Biochem/physiol Actions

Also contains proteolytic activity.

Packaging

25, 100 units in glass bottle

Unit Definition

One unit will convert 1.0 μmole of xanthine to uric acid per min at pH 7.5 at 25 °C. Approx. 50% of the activity is obtained with hypoxanthine as substrate.

Physical form

Lyophilized powder containing BSA and sodium glutamate as stabilizers

General description

Allopurinol is a synthetic drug show to inhibit xanthine oxidase.

Physical properties

Inhibitors: Reducing agents, Hg++, Ag+,MIA
Optimum pH: 7.5 - 8.0
Optimum temperature: 65oC
pH Stability: pH 6.5 - 9.0 (25oC, 15hr)
Thermal stability: below 55oC (pH 8.0, 30min)

Price and Availability


All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
RQ8455000
Protocols & Articles

Protocols

Assay Procedure for Xanthine Oxidase Microbial

One unit causes the formation of one micromole of uric acid per minute under the conditions described below.
Keywords: Extinction coefficient

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Molecular biology

Peer-Reviewed Papers
15

References

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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