X4126 Sigma

Xylene Cyanol FF

for molecular biology, BioReagent

Synonym: Acid blue 147, Cyanol FF, XC



General description

Xylene cyanol is often used as a tracking dye during agarose or polyacrylamide gel electrophoresis. It has a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition.


10 g in glass bottle


A 6x Gel Loading Buffer can be made using standard recipes, including 0.05% xylene cyanol.


Xylene Cyanol FF is used as histological stain and as tracker dye in DNA sequencing and electrophoresis.1,2

Biochem/physiol Actions

Xylene Cyanol FF acts as nucleic acid stain.

Price and Availability


Customers Also Viewed

Bromophenol Blue sodium salt

for molecular biology, for electrophoresis

for NA electrophoresis, solution

Orange G

for NA electrophoresis

Safety & Documentation

Safety Information

GHS07  GHS07
Signal word 
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
WGK Germany 


Certificate of Analysis

Certificate of Origin

Protocols & Articles


Introduction to Nucleic Acid Electrophoresis

Introduction Agarose gel electrophoresis for DNA Agarose gel electrophoresis for RNA Polyacrylamide gel electrophoresis for DNA Reference
Keywords: AGE, Buffers, Degradations, Electrophoresis, Environmental, Gel electrophoresis, PAGE, Purification, Separation, Size-exclusion chromatography

Peer-Reviewed Papers


Set your institution to view full text papers.

1. Analysis of estrogen receptor polymorphism in codon 325 by PCR-SSCP in breast cancer: association with lymph node metastasis. Vasconcelos A, Medeiros R, Veiga I, et al. Breast J. 8(4), 226-9, (2002)


2. Methods and reagents. Agarose gel electrophoresis in your kitchen. Hengen PN Trends Biochem. Sci. 20(5), 202-3, (1995)


Incorporation of a viral DNA-packaging motor channel in lipid bilayers for real-time, single-molecule sensing of chemicals and double-stranded DNA. Haque F, Geng J, Montemagno C, et al. Nat. Protoc. 8(2), 373-92, (2013)


A new method for sequencing DNA. Maxam, A.M. and Gilbert, W Proc. Natl. Acad. Sci. U. S. A. 74, 560-564, (1977)


Electrophoresis in agarose and acrylamide gels. Ogden, R.C. and Adams, D.A., Meth. Enzymol. 152, 61-87, (1987)


Bleach gel: a simple agarose gel for analyzing RNA quality. Aranda PS, LaJoie DM, Jorcyk CL. Electrophoresis 33, 366-369, (2012)


A strong-stop DNA in rice plants infected with rice tungro bacilliform virus. Bao Y and Hull R J. Gen. Virol. 74 ( Pt 8), 1611-6, (1993)


Association between anti-U1 ribonucleoprotein antibodies and inflammatory mediators in cerebrospinal fluid of patients with neuropsychiatric systemic lupus erythematosus. Yokoyama T, Fujii T, Kondo-Ishikawa S, et al. Lupus 23(7), 635-642, (2014)


Free-radical first responders: the characterization of CuZnSOD and MnSOD regulation during freezing of the freeze-tolerant North American wood frog, Rana sylvatica. Dawson NJ, Katzenback BA, Storey KB, et al. Biochim. Biophys. Acta 1850(1), 97-106, (2014)


Cerebral angioplasty using the Scepter XC dual lumen balloon for the treatment of vasospasm following intracranial aneurysm rupture. Heit JJ, Choudhri O, Marks MP, et al. J. Neurointerv. Surg. 7(1), 56-61, (2014)


Effects of genetic variation and growing condition of prairie cordgrass on feedstock composition and ethanol yield. Kim SM, Guo J, Kwak S, et al. Bioresour. Technol. 183, 70-7, (2015)


SKP2 cooperates with N-Ras or AKT to induce liver tumor development in mice. Delogu S, Wang C, Cigliano A, et al. Oncotarget 6(4), 2222-34, (2015)


Endovascular treatment of 346 middle cerebral artery aneurysms: results of a 16-year single-center experience. Kadkhodayan Y, Delgado Almandoz JE, Fease JL, et al. Neurosurgery 76(1), 54-60; discussion 60-1, (2014)


Dicer nuclease-promoted production of Let7a-1 microRNA is enhanced in the presence of tryptophan-containing amphiphilic peptides. Hyun S, Han A, Jo MH, et al. ChemBioChem. 15(11), 1651-9, (2014)


Microglial content-dependent inhibitory effects of calcitonin gene-related peptide (CGRP) on murine retroviral infection of glial cells. Malon JT, Grlickova-Duzevik E, Vaughn J, et al. J. Neuroimmunol. 279, 64-70, (2015)


Linkage analysis and map construction in genetic populations of clonal F1 and double cross. Zhang L, Li H, Wang J, et al. G3 (Bethesda) 5(3), 427-39, (2015)


Identification of bacteria pathogenic to or associated with onion (Allium cepa) based on sequence differences in a portion of the conserved gyrase B gene. Bonasera JM, Asselin JA, Beer SV, et al. J. Microbiol. Methods 103, 138-43, (2014)


Enzymatic primer-extension with glycerol-nucleoside triphosphates on DNA templates. Chen JJ, et al. PLoS ONE 4(3), e4949, (2009)


Beil. 14,II,514

Related Products

related product

Product #


Add to Cart

D5282 Lambda DNA EcoR I Digest, for DNA electrophoresis
D5042 123 bp DNA Ladder, for DNA electrophoresis
D0428 1 kb DNA Ladder, for DNA electrophoresis
S7025 50 bp DNA Step Ladder, for DNA electrophoresis
A6014 Acridine Orange hemi(zinc chloride) salt, For nucleic acid staining in cells or gels
O3756 Orange G, for NA electrophoresis
B5525 Bromophenol Blue sodium salt
M4159 Methylene Blue hydrate, suitable for nucleic acid staining, BioReagent

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?