|Related Categories||In Vivo/In Vitro Tracking of Stem Cells, Assays and Reagents for Measuring Cytotoxicity, Proliferation and Viability, Cell Biology, Cell Culture, Cell Viability,|
|solubility||H2O: soluble2.5 mg/mL (hot)|
|suitability||suitable for cell culture|
XTT has been used in conjunction with phenazine methosulfate (PMS) to screen human cancer cell lines.1 The tetrazolium dye has also been used to study fungal cell damage,2 in testing antimicrobial susceptibility of staphylococci,3 and in Candida biofilm research.4 Limitations to the XTT assay have been reported.5
In living cells, XTT is metabolically reduced to produce a colorimetric, water-soluble formazan product.
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for quantitation of viable cell number in proliferation and cytotoxicity assays
powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥97.5% (HPLC)
3. Comparative evaluation of 2,3-bis [2-methyloxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide (XTT) and 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2, 4-disulfophenyl)-2H-tetrazolium, monosodium salt (WST-8) rapid colorimetric assays for antimicrobial susceptibility testing of staphylococci and ESBL-producing clinical isolates. Brady, A.J., et al. J. Microbiol. Methods 71, 305-11, (2007)
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