EMAIL THIS PAGE TO A FRIEND

X4626 Sigma

XTT sodium salt

powder, BioReagent, suitable for cell culture

Synonym: 2,3-Bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide inner salt

Purchase

Description

Application

XTT has been used in conjunction with phenazine methosulfate (PMS) to screen human cancer cell lines.3 The tetrazolium dye has also been used to study fungal cell damage,4 in testing antimicrobial susceptibility of staphylococci,5 and in Candida biofilm research.6 Limitations to the XTT assay have been reported.7

Principle

In living cells, XTT is metabolically reduced to produce a colorimetric, water-soluble formazan product.

Biochem/physiol Actions

XTT (sodium 3′-[1-[(phenylamino)-carbony]-3,4-tetrazolium]-bis(4-methoxy-6-nitro)benzene-sulfonic acid hydrate) is acted upon by dehydrogenases present in metabolically active cells. This produces water soluble formazan, which is a highly colored product. Thus, it offers an advantage in colorimetric proliferation assays, as opposed to MTT, as the product is already soluble and no formazan crystals are formed.1

General description

XTT (sodium 3′-[1-[(phenylamino)-carbony]-3,4-tetrazolium]-bis(4-methoxy-6-nitro)benzene-sulfonic acid hydrate) is a tetrazolium salt, which forms water-soluble formazan on bioreduction.2

Price and Availability


Learn about PharmaGrade

Customers Also Viewed

for quantitation of viable cell number in proliferation and cytotoxicity assays

Menadione

crystalline

MTS-4-Fluorescein

≥90% (TLC)

Phenazine methosulfate

≥90% (UV)

Safety & Documentation

Safety Information

WGK Germany 
3

Protocols & Articles

Peer-Reviewed Papers

References

Set your institution to view full text papers.

1. An improved colorimetric assay for cell proliferation and viability utilizing the tetrazolium salt XTT. Roehm, N., et al. J. Immunol. Methods 142, 257-265, (1991)

Loading...


2. Improved short- and long-term XTT-based colorimetric cellular cytotoxicity assay for melanoma and other tumor cells. Jost LM, Kirkwood JM, and Whiteside TL J. Immunol. Methods 147(2), 153-65, (1992)

Loading...


3. Evaluation of a soluble tetrazolium/formazan assay for cell growth and drug sensitivity in culture using human and other tumor cell lines. Scudievo, et al. Cancer Res. 48, 4827, (1988)

Loading...


5. Comparative evaluation of 2,3-bis [2-methyloxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide (XTT) and 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2, 4-disulfophenyl)-2H-tetrazolium, monosodium salt (WST-8) rapid colorimetric assays for antimicrobial susceptibility testing of staphylococci and ESBL-producing clinical isolates. Brady, A.J., et al. J. Microbiol. Methods 71, 305-11, (2007)

Loading...


6. Improvement of XTT assay performance for studies involving Candida albicans biofilms. da Silva, W.J., et al. Braz. Dent. J. 19, 264-9, (2008)

Loading...


7. Uses and limitations of the XTT assay in studies of Candida growth and metabolism. Kuhn, D.M., et al. J. Clin. Microbiol. 41, 506-8, (2003)

Loading...


A water-soluble tetrazolium salt useful for colorimetric cell viability assay. Tominaga, H., et al. Anal. Comm. 36, 47-50, (1999)

Loading...


Related Products

related product

Product #

Description

Add to Cart

TOX2 In Vitro Toxicology Assay Kit, XTT based
08168 Timestrip Plus 8 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?