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XNABR Sigma

REDExtract-N-Amp Blood PCR Kit

sufficient for 1000 extractions, sufficient for 1000 amplifications

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Properties

Related Categories Cell Biology, DNA & RNA Purification, General Stem Cell Biology, Genomic DNA Amplification, Genomic DNA Purification,
usage   sufficient for 1000 amplifications
  sufficient for 1000 extractions
feature   hotstart
color   red
shipped in   wet ice
storage temp.   −20°C

Description

Features and Benefits

• Efficient 8 minute prep allows greater speed and throughput
• No need for organic extraction, centrifugation or alcohol precipitation
• Hot start antibody for highly specific PCR amplification of genomic DNA
• Can be used with whole blood or blood cards
• Extract stable at 4 °C for at least 6 months

Other Notes

For additional information, please see www.sigma-aldrich.com/extract-n-amp.

Legal Information

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Application

REDExtract-N-Amp Blood PCR Kit has been used to extract genomic DNA from blood samples, peripheral blood leukocytes, parasites and endocervical swab eluates of mice. It has also been used to perform polymerase chain reaction (PCR) using oligonucleotide primers.

Principle

Genomic DNA is extracted from 10 μl of whole blood by simply adding the Extraction Solution and incubating for 5 minutes at room temperature. The Neutralization Solution is added to the extract to counteract inhibitory substances prior to PCR. A portion of the DNA extract is then added to the specially formulated PCR ReadyMix.

General description

The REDExtract-N-Amp Blood PCR Kits contain all of the reagents necessary to rapidly extract host genomic DNA from whole blood and amplify targets of interest by PCR. This novel extraction system eliminates the need for any type of purification, organic extraction, centrifugation, heating, filtration or alcohol precipitation. The kit also includes a PCR ReadyMix, specially formulated for amplification directly from the extract. This formulation uses an antibody based hot start, for specific amplification. The REDExtract-N-Amp Blood PCR Kit contains a tracking dye that allows for convenient direct loading of PCR reactions onto agarose gels for analysis.

Price and Availability


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Microcon Centrifugal Filters

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Safety & Documentation

Safety Information

Symbol 
GHS05  GHS05
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN 3316 9
Protocols & Articles

Articles

Extract-N-Amp Reagent Provides a Highly Effective Way of Generating PCR Products from FTA® Blood Cards

Extract-N-Amp is a versatile, combined DNA extraction and amplification kit intended to simplify the generation of PCR products from a range of sample types. As shown in this article, this methodolog...
Mairi J. Hunter, Iain McLaren-Lee, Nicholas R. Helps
LSI Edition 23
Keywords: Amplification, Polymerase chain reaction, Polymorphisms, Sample preparations, Sequencing, Size-exclusion chromatography

Extract-N-Amp™ Blood Product Information

The Extract-N-Amp Blood PCR kits contain all of the reagents necessary to rapidly extract genomic DNA from whole blood and amplify targets of interest by PCR (Figure 1).
Keywords: Amplification, Centrifugation, Cloning, DNA purification, Digestions, Filtration, Polymerase chain reaction, Precipitation, Purification, Sequencing

Extract-N-Amp™ Product Guide

Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays. Eliminate the need for any columns or long enzymatic sample puri...
Keywords: Amplification, Buffers, Digestions, Homogenization, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, Sequencing

Sample Purification & Quality Assessment - PCR Technologies Guide

The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RN...
Keywords: AGE, Absorption, Amplification, Bio-Analytical, Buffers, Capillary electrophoresis, Cell disruption, Centrifugation, Chromatin immunoprecipitation, DNA purification, Degradations, Detection methods, Detergents, Digestions, Electrophoresis, Extinction coefficient, Filtration, Gel electrophoresis, Gene expression, Indicators, Lab-on-a-chip, Molecular biology, Nucleic acid hybridization, Nucleic acid purification, Polymerase chain reaction, Polymerase chain reaction - quantitative, Precipitation, Purification, RNA purification, Separation, Spectroscopy, Transcription, Ultraviolet-Visible spectroscopy

Protocols

REDExtract-N-Amp™ Blood PCR Kits Protocol

Product Description Storage Reagents and equipment required but not provided Precautions and Disclaimer Procedure PCR amplification Troubleshooting Guide Materials References
Keywords: Amplification, Coagulation, Diagnostic, Evaporation, Gas chromatography, Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction, Sequencing, Solvents

Related Content

Automated Protocols for Life Science Research

Scientists at Sigma-Aldrich® have validated all of the automated methods available below. Automation protocols are also provided as instruction for each of the methods. These protocols supply informa...
Keywords: Amplification, Polymerase chain reaction, Polymerase chain reaction - quantitative

PCR Selection Guide

Sigma-Aldrich offers a wide variety of PCR reagents to meet any experimental needs. Our range of polymerases is customized to meet your End-Point PCR, qPCR, or RT-PCR needs. Our products vary from ro...
Keywords: Polymerase chain reaction, Polymerase chain reaction - quantitative

Peer-Reviewed Papers
15

References

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L3289 Lysis solution for blood
N9784 Neutralization Solution for Blood
06693 Timestrip Plus -20 °C

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