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XNAP2 Sigma

Extract-N-Amp Plant PCR Kit

sufficient for 100 extractions, sufficient for 100 amplifications

Purchase

Properties

Related Categories DNA & RNA Purification, Extract-N-Amp Plant PCR Kits, Genome Mapping and Genotyping, Genomic DNA Amplification, Genomic DNA Purification,
usage   sufficient for 100 amplifications
  sufficient for 100 extractions
feature   hotstart
color   colorless
Featured Industry   Agriculture
shipped in   wet ice
storage temp.   −20°C

Description

Components

Kits contain Dilution solution, Extraction solution, and Extract-N-Amp PCR Reaction Mix.

Other Notes

For additional information, please see www.sigma-aldrich.com/extract-n-amp.

Legal Information

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Application

Extract-N-Amp Plant PCR Kit has been used for genomic DNA isolation from the fungal mycelium.1

Features and Benefits

• Single-step extraction of plant genomic DNA for PCR in less than 15 minutes
• No freezing, mechanical disruption, organic extraction, column purification or precipitation required
• Specially formulated PCR ReadyMix for use with extract
• Hot Start antibody for highly specific PCR amplification of genomic DNA
• REDExtract-N-Amp requires no loading buffers or tracking dyes required for gel analysis
• Compatible with high-throughput requirements for genetic analysis of plants
• Extract stable at 4 °C for at least 6 months

General description

The Extract-N-Amp Plant PCR Kits contain all the reagents necessary to rapidly extract genomic DNA from plant leaves and amplify targets of interest by PCR. A novel Extraction Solution eliminates the need for conventional freezing of plant tissues with liquid nitrogen, mechanical disruption, organic extraction, column purification, or precipitation of DNA.

Price and Availability

PCR Selection Guide


PCR Selection GuideNeed help choosing between Taq® and ReadyMix™ products?

Use our interactive PCR selection guide to help you choose the right product for your research needs.




PCR Technical Manual

Genotyping

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Extract-N-Amp Plant Dilution Solution

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sufficient for 100 extractions, sufficient for 500 amplifications

sufficient for 1000 extractions, sufficient for 1000 amplifications

REDExtract-N-Amp<SUP>™</SUP> Plant PCR Kit

sufficient for 10 extractions, sufficient for 10 amplifications

REDExtract-N-Amp<SUP>™</SUP> Plant PCR Kit

sufficient for 100 extractions, sufficient for 100 amplifications

Safety & Documentation

Protocols & Articles

Articles

Extract-N-Amp™ Plant PCR Kit

Description Procedure Application Features and Benefits Order Information Kit Contents Sample Data Materials
Keywords: Amplification, DNA purification, Digestions, Phase transitions, Polymerase chain reaction, Precipitation, Purification

Extract-N-Amp™ Plant Product Information

Description Procedure Application Features and Benefits Order Information Kit Contents Sample Data
Keywords: Amplification, DNA purification, Digestions, Phase transitions, Polymerase chain reaction, Precipitation, Purification

Extract-N-Amp™ Product Guide

Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays. Eliminate the need for any columns or long enzymatic sample puri...
Keywords: Amplification, Buffers, Digestions, Homogenization, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, Sequencing

Improving Real-Time PCR Success

To meet increasing food supply demands of the global population, growers require seeds that can withstand the regional challenges found in their fields. In order to develop crop varieties that are mo...
Keywords: Agriculture, Amplification, Environmental, Polymerase chain reaction

Sample Purification & Quality Assessment - PCR Technologies Guide

The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RN...
Keywords: AGE, Absorption, Amplification, Bio-Analytical, Buffers, Capillary electrophoresis, Cell disruption, Centrifugation, Chromatin immunoprecipitation, DNA purification, Degradations, Detection methods, Detergents, Digestions, Electrophoresis, Extinction coefficient, Filtration, Gel electrophoresis, Gene expression, Indicators, Lab-on-a-chip, Molecular biology, Nucleic acid hybridization, Nucleic acid purification, Polymerase chain reaction, Polymerase chain reaction - quantitative, Precipitation, Purification, RNA purification, Separation, Spectroscopy, Transcription, Ultraviolet-Visible spectroscopy

Protocols

Extract-N-Amp™ Plant PCR Kits Protocol

Product Description Reagents and Equipment Required But Not Provided Precautions and Disclaimer Storage Procedure Troubleshooting Guide Materials References
Keywords: Amplification, Diagnostic, Evaporation, Gas chromatography, Nucleic acid annealing, Nucleic acid denaturation, Phase transitions, Polymerase chain reaction, Precipitation, Purification, Sequencing

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Sigma-Aldrich’s chemistry expertise can help customers develop and optimize their entire genotyping workflow to reduce cost per data point while increasing throughput rates. In addition, our global d...
Keywords: Amplification, Polymerase chain reaction, Polymerase chain reaction - quantitative, Sequencing, Whole genome amplification

Agriculture | Plant Breeding Workflow

Sigma-Aldrich products are aligned to the discovery, development and production phases of the plant breeding workflow, allowing you to develop and produce new crop varieties faster. With materials ma...
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Peer-Reviewed Papers

References

Set your institution to view full text papers.

1. Signaling by the pathogenicity-related MAP kinase of Cochliobolus heterostrophus correlates with its local accumulation rather than phosphorylation. Lev S, Tal H, Rose MS, et al. Mol. Plant Microbe Interact. 22(9), 1093-103, (2009)

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Chloroplast ribonucleoprotein CP31A is required for editing and stability of specific chloroplast mRNAs. Tillich M, Hardel SL, Kupsch C, et al. Proc. Natl. Acad. Sci. U. S. A. 106(14), 6002-7, (2009)

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A protocol for the rapid isolation of full geminivirus genomes from dried plant tissue. Shepherd DN, Martin DP, Lefeuvre P, et al. J. Virol. Methods 149(1), 97-102, (2008)

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Spontaneous spectinomycin resistance mutations detected after biolistic transformation of Daucus carota L. Filipenko EA, Sidorchuk YV, Titov II, et al. Physiol. Mol. Biol. Plants 17(1), 79-86, (2011)

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A High-Throughtput System for the Rapid Extraction of Plant Genomic DNA for Genome Mapping and Marker-Assited Breeding Studies Wang, D., et al. J. Assoc. Lab. Autom. 10, 242, (2005)

A direct PCR approach to accelerate analyses of human-associated microbial communities. Flores GE, Henley JB, and Fierer N PLoS ONE 7(9), e44563, (2012)

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Altered starch turnover in the maternal plant has major effects on Arabidopsis fruit growth and seed composition. Andriotis VM, Pike MJ, Schwarz SL, et al. Plant Physiol. 160(3), 1175-86, (2012)

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The ectomycorrhizal fungal community in a neotropical forest dominated by the endemic dipterocarp Pakaraimaea dipterocarpacea. Smith ME, Henkel TW, Uehling JK, et al. PLoS ONE 8(1), e55160, (2013)

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Expression, purification, and characterization of recombinant human transferrin from rice (Oryza sativa L.). Zhang D, Nandi S, Bryan P, et al. Protein Expr. Purif. 74(1), 69-79, (2010)

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Mycorrhizal response to experimental pH and P manipulation in acidic hardwood forests. Kluber LA, Carrino-Kyker SR, Coyle KP, et al. PLoS ONE 7(11), e48946, (2012)

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Knocking-down Meloidogyne incognita proteases by plant-delivered dsRNA has negative pleiotropic effect on nematode vigor. Antonino de Souza Júnior JD, Ramos Coelho R, Tristan Lourenço I, et al. PLoS ONE 8(12), e85364, (2013)

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Tissue specificity and evolution of meristematic WOX3 function. Rena Shimizu et al Plant Physiol. 149, 841-50, (2009)

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