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XNAPR Sigma

REDExtract-N-Amp Plant PCR Kit

sufficient for 1000 extractions, sufficient for 1000 amplifications

Purchase

Properties

Related Categories Automation Reagents, DNA & RNA Purification, Extract-N-Amp Plant PCR Kits, Genome Mapping and Genotyping, Genomic DNA Amplification,
usage   sufficient for 1000 amplifications
  sufficient for 1000 extractions
feature   hotstart
color   red
Featured Industry   Agriculture
storage temp.   −20°C

Description

Components

Kits contain Dilution solution, Extraction solution, and REDExtract-N-Amp PCR Reaction Mix.

Other Notes

For additional information, please see www.sigma-aldrich.com/extract-n-amp.

Legal Information

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Application

REDExtract-N-Amp Plant PCR Kit has been used to extract genomic DNA from leaves and mycelial lawns. It has also been used for polymerase chain reaction.

Features and Benefits

• Single-step extraction of plant genomic DNA for PCR in less than 15 minutes
• No freezing, mechanical disruption, organic extraction, column purification or precipitation required
• Specially formulated PCR ReadyMix for use with extract
• Hot Start antibody for highly specific PCR amplification of genomic DNA
• REDExtract-N-Amp requires no loading buffers or tracking dyes required for gel analysis
• Compatible with high-throughput requirements for genetic analysis of plants
• Extract stable at 4 °C for at least 6 months (Fig. 3)

General description

The REDExtract-N-Amp Plant PCR Kits contain all the reagents necessary to rapidly extract genomic DNA from plant leaves and amplify targets of interest by PCR. The REDExtract-N-Amp PCR ReadyMix contains a dye that acts as a tracking dye and allows for convenient direct loading of PCR reactions onto agarose gels for analysis.

Price and Availability


Microcon Centrifugal Filters

All labs need water
Protocols & Articles

Articles

Extract-N-Amp™ Plant Product Information

Description Procedure Application Features and Benefits Order Information Kit Contents Sample Data
Keywords: Amplification, DNA purification, Digestions, Phase transitions, Polymerase chain reaction, Precipitation, Purification

Extract-N-Amp™ Plant Tissue PCR Kits

Description Procedure Application Features and Benefits Order Information Kit Contents Sample Data Materials
Keywords: Amplification, DNA purification, Digestions, Phase transitions, Polymerase chain reaction, Precipitation, Purification

Extract-N-Amp™ Product Guide

Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays. Eliminate the need for any columns or long enzymatic sample puri...
Keywords: Amplification, Buffers, Digestions, Homogenization, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, Sequencing

Improving Real-Time PCR Success

To meet increasing food supply demands of the global population, growers require seeds that can withstand the regional challenges found in their fields. In order to develop crop varieties that are mo...
Keywords: Agriculture, Amplification, Environmental, Polymerase chain reaction

Sample Purification & Quality Assessment - PCR Technologies Guide

The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RN...
Keywords: AGE, Absorption, Amplification, Bio-Analytical, Buffers, Capillary electrophoresis, Cell disruption, Centrifugation, Chromatin immunoprecipitation, DNA purification, Degradations, Detection methods, Detergents, Digestions, Electrophoresis, Extinction coefficient, Filtration, Gel electrophoresis, Gene expression, Indicators, Lab-on-a-chip, Molecular biology, Nucleic acid hybridization, Nucleic acid purification, Polymerase chain reaction, Polymerase chain reaction - quantitative, Precipitation, Purification, RNA purification, Separation, Spectroscopy, Transcription, Ultraviolet-Visible spectroscopy

Solving the Space Constraints of High-Throughput Genotyping

High-throughput genotyping is a critical tool for modern plant breeding programs. The more samples analyzed, the more marker data collected, and the better the chances to identify traits that enhance...
Keywords: Agriculture, Sample preparations

Protocols

REDExtract-N-Amp™ Plant PCR Kits Protocol

Product Description Reagents and Equipment Required But Not Provided Precautions and Disclaimer Storage Procedure Related Products Troubleshooting Guide Materials References
Keywords: Amplification, Diagnostic, Evaporation, Gas chromatography, Nucleic acid annealing, Nucleic acid denaturation, Phase transitions, Polymerase chain reaction, Precipitation, Purification, Sequencing

Related Content

Agriculture | Genotyping

Sigma-Aldrich’s chemistry expertise can help customers develop and optimize their leaf and seed genotyping workflow to reduce cost per data point while increasing throughput rates. In addition, our g...
Keywords: Amplification, Polymerase chain reaction, Polymerase chain reaction - quantitative, Sequencing, Whole genome amplification

Automated Protocols for Life Science Research

Scientists at Sigma-Aldrich® have validated all of the automated methods available below. Automation protocols are also provided as instruction for each of the methods. These protocols supply informa...
Keywords: Amplification, Polymerase chain reaction, Polymerase chain reaction - quantitative

PCR Selection Guide

Sigma-Aldrich offers a wide variety of PCR reagents to meet any experimental needs. Our range of polymerases is customized to meet your End-Point PCR, qPCR, or RT-PCR needs. Our products vary from ro...
Keywords: Polymerase chain reaction, Polymerase chain reaction - quantitative

Plant Breeding Workflow - Collaborating to Empower Yield Improvements

Sigma-Aldrich products are aligned to the discovery, development and production phases of the plant breeding workflow, allowing you to develop and produce new crop varieties faster. With materials ma...

Peer-Reviewed Papers
15

References

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06693 Timestrip Plus -20 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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