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XNAT2R Sigma

Extract-N-Amp Tissue PCR Kit

sufficient for 1000 extractions, sufficient for 1000 amplifications

Purchase

Properties

Related Categories Automation Reagents, Cell Biology, DNA & RNA Purification, General Stem Cell Biology, Genomic DNA Amplification,
usage   sufficient for 1000 amplifications
  sufficient for 1000 extractions
feature   hotstart
color   colorless
shipped in   wet ice
storage temp.   −20°C

Description

Components

Kits contain Tissue Preparation Solution, Extraction Solution, and Extract-N-Amp PCR Reaction Mix.

Features and Benefits

• Perfect for quick genomic DNA isolation for genotyping
• Single-step extraction of genomic DNA for PCR in 15 minutes
• PCR ReadyMix is specially formulated for amplification directly from extract
• Hot Start antibody for highly specific PCR amplification of genomic DNA
• Extract stable at 4 oC for at least 6 months

Other Notes

For additional information, please see www.sigma-aldrich.com/extract-n-amp.

Legal Information

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Application

Extract-N-Amp tissue PCR kit has been used for DNA extraction and polymerase chain reaction (PCR).

Principle

The kit contains validated protocols to extract and amplify genomic DNA from mouse-tails, hair, animal tissue, saliva, and buccal swabs. In a typical procedure, genomic DNA is extracted from a sample that has been incubated in the tissue preparation solution and extraction solution for 10 minutes at room temperature. The sample is heated to 95 °C for 3 minutes and then mixed with a third solution to neutralize inhibitory substances prior to PCR. A portion of the DNA extract is then added to a PCR reaction containing primers and Extract-N-Amp PCR ReadyMix, included in the kit.

General description

The Extract-N-Amp Tissue PCR Kits provide all the reagents necessary to rapidly extract DNA from a wide variety of cells and tissues and amplify targets of interest by PCR.

Price and Availability


Your Key to Success

Microcon Centrifugal Filters

All labs need water
Safety & Documentation
Protocols & Articles

Articles

DNA & RNA Purification Extract-N-Amp Tissue

The Extract-N-Amp™ Tissue PCR Kits provide all the reagents necessary to rapidly extract DNA from a wide variety of cells and tissues and amplify targets of interest by PCR (Figure 1).
Keywords: Amplification, Digestions, Homogenization, Polymerase chain reaction, Polymerase chain reaction - quantitative

Extract-N-Amp™ Product Guide

Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays. Eliminate the need for any columns or long enzymatic sample puri...
Keywords: Amplification, Buffers, Digestions, Homogenization, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, Sequencing

Extract-N-Amp™ Tissue Feature Article By: Scott Weber and Derek Douglas

Introduction Materials and Methods Results and Discussion Conclusions Acknowledgements References Ordering Information
Scott Weber and Derek Douglas
Sigma-Aldrich Corporation, St. Louis, MO, USA
Keywords: Amplification, Digestions, Polymerase chain reaction, Polymerase chain reaction - quantitative, Precipitation, Purification, Sequencing

Frequently asked questions - Extract-N-Amp™ Tissue PCR Kit

Can the isolated DNA be used in applications other than PCR such as restriction digestion or Southern blots? Can the PCR product amplified with the Extract-N-Amp Tissue PCR Kit be used for other down...
Keywords: Amplification, Digestions, Polymerase chain reaction, Polymerase chain reaction - quantitative, Sequencing

Sample Purification & Quality Assessment - PCR Technologies Guide

The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RN...
Keywords: AGE, Absorption, Amplification, Bio-Analytical, Buffers, Capillary electrophoresis, Cell disruption, Centrifugation, Chromatin immunoprecipitation, DNA purification, Degradations, Detection methods, Detergents, Digestions, Electrophoresis, Extinction coefficient, Filtration, Gel electrophoresis, Gene expression, Indicators, Lab-on-a-chip, Molecular biology, Nucleic acid hybridization, Nucleic acid purification, Polymerase chain reaction, Polymerase chain reaction - quantitative, Precipitation, Purification, RNA purification, Separation, Spectroscopy, Transcription, Ultraviolet-Visible spectroscopy

Protocols

Extract-N-Amp™ Tissue PCR Kit Protocol

Product Description Reagents and Equipment Required But Not Provided Precautions and Disclaimer Storage Procedure PCR amplification Troubleshooting Guide Materials References
Keywords: Amplification, Diagnostic, Evaporation, Gas chromatography, Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction, Precipitation, Purification, Sequencing

Related Content

Automated Protocols for Life Science Research

Scientists at Sigma-Aldrich® have validated all of the automated methods available below. Automation protocols are also provided as instruction for each of the methods. These protocols supply informa...
Keywords: Amplification, Polymerase chain reaction, Polymerase chain reaction - quantitative

PCR Selection Guide

Sigma-Aldrich offers a wide variety of PCR reagents to meet any experimental needs. Our range of polymerases is customized to meet your End-Point PCR, qPCR, or RT-PCR needs. Our products vary from ro...
Keywords: Polymerase chain reaction, Polymerase chain reaction - quantitative

Peer-Reviewed Papers
15

References

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92210 Timestrip Plus 0 °C
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