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XNAT Sigma

REDExtract-N-Amp Tissue PCR Kit

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Properties

Related Categories Cell Biology, DNA & RNA Purification, General Stem Cell Biology, Genomic DNA Amplification, Genomic DNA Purification,
usage   10 reactions sufficient for 10 amplifications
  10 reactions sufficient for 10 extractions
  100 reactions sufficient for 100 amplifications
  100 reactions sufficient for 100 extractions
  1000 reactions sufficient for 1000 amplifications
  1000 reactions sufficient for 1000 extractions
feature   hotstart
color   red
shipped in   wet ice
storage temp.   −20°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Components

Kits contain Tissue Preparation Solution, Extraction Solution, and REDExtract-N-Amp PCR Reaction Mix.

Features and Benefits

• Perfect for quick genomic DNA isolation for genotyping
• Single-step extraction of genomic DNA for PCR in 15 minutes
• PCR ReadyMix, specially formulated for amplification directly from extract
• Hot Start antibody for highly specific PCR amplification of genomic DNA
• Extract stable at 4 oC for at least 6 months

Other Notes

The REDExtract-N-Amp Tissue PCR Kits provide all the reagents necessary to rapidly extract DNA from a wide variety of cells and tissues and amplify targets of interest by PCR. A novel extraction method eliminates the need for long enzymatic digestions or homogenization. The kit also includes a specially formulated hot start PCR reaction mix for amplification directly from the extract. The REDExtract-N-Amp PCR ReadyMix contains an inert dye that acts as a tracking dye and allows for convenient loading of PCR reactions onto agarose gels for analysis.

For additional information, please see www.sigma-aldrich.com/extract-n-amp.

Legal Information

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Application

REDExtract-N-Amp tissue PCR kit has been used for DNA extraction and in polymerase chain reaction (PCR).

Principle

The kit comes with validated protocols to extract and amplify genomic DNA from mouse-tails, hair, animal tissue, saliva, and buccal swabs. In a typical procedure, genomic DNA is extracted from a sample that has been incubated in the tissue preparation solution and extraction solution for 10 minutes at room temperature. The sample is heated to 95 °C for 3 minutes and then mixed with a third solution to neutralize inhibitory substances prior to PCR. A portion of the DNA extract is then added to a PCR reaction containing primers and REDExtract-N-Amp PCR ReadyMix, included in the kit.

Price and Availability


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Safety & Documentation

Safety Information

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Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Frequently Asked Questions

How do I quantitate my DNA with XNAT, REDExtract-N-Amp® Tissue PCR Kit?
The DNA extract generated using the Extract-N-Amp kit is very crude, and it is not possible to obtain a reliable 260/280 ratio.  For quantitation of pure DNA, we recommend using one of our GenElute genomic DNA kits.
How do I store my DNA samples for use with XNAT, REDExtract-N-Amp®  Tissue PCR Kit?
DNA samples can be stored for up to 6 months at 2-8 deg C with the tissue sample removed.
Are the XNAT, REDExtract-N-Amp® Tissue PCR Kit components available separately?
Only R4775, the REDExtract-N-Amp PCR Reaction Mix is available separately.
What is the shelf life of the kit XNAT, REDExtract-N-Amp® Tissue PCR Kit and how should I store it?
The shelf life of XNAT has not been determined.  However, based on our storage guidelines, it should be usable for 1 year from the date of receipt.  Click on the following link to view Shipping/Storage/Shelf Life/Expiration date statement: <rn:answer_xref style="TEXT-INDENT: 0in" title="Product Dating" target="_self" contents="http://www.sigmaaldrich.com/content/dam/sigma-aldrich/docs/Sigma-Aldrich/General_Information/product-dating-information-statement-v5.pdf" answer_id="89" />                                      Storage conditions are found in XNAT Technical Bulletin.  The REDExtract-N-Amp Tissue PCR Kit can be stored at 2-8°C for up to three weeks. For long-term storage, greater than three weeks, -20°C is recommended. Do not store in a ""frost-free"" freezer.  We recommend to store in 10 reaction aliquots.  Click on the following link to view XNAT Technical Bulletin: http://www.sigmaaldrich.com/sigma/bulletin/xnatbul.pdf.
I am unable to detect any PCR product with Product XNAT, REDExtract-N-Amp® Tissue PCR Kit.
From XNAT Technical Bulletin, troubleshooting guide table:  PCR reaction may be inhibited due to contaminants in the tissue extract.  Dilute the tissue extract with a 50:50 mix of extraction and neutralization solutions.  To test for inhibition, include a DNA control and/or spike a known amount of template (100-500 copies) into the PCR along with the tissue extract.  Click on the following link to view XNAT Technical Bulletin:  http://www.sigmaaldrich.com/sigma/bulletin/xnatbul.pdf
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
Show more questions
Protocols & Articles

Articles

DNA & RNA Purification Extract-N-Amp Tissue

The Extract-N-Amp™ Tissue PCR Kits provide all the reagents necessary to rapidly extract DNA from a wide variety of cells and tissues and amplify targets of interest by PCR (Figure 1).
Keywords: Amplification, Digestions, Homogenization, Polymerase chain reaction, Polymerase chain reaction - quantitative

Extract-N-Amp™ Product Guide

Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays. Eliminate the need for any columns or long enzymatic sample puri...
Keywords: Amplification, Buffers, Digestions, Homogenization, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, Sequencing

Extract-N-Amp™ Tissue Feature Article By: Scott Weber and Derek Douglas

Introduction Materials and Methods Results and Discussion Conclusions Acknowledgements References Ordering Information
Scott Weber and Derek Douglas
Sigma-Aldrich Corporation, St. Louis, MO, USA
Keywords: Amplification, Digestions, Polymerase chain reaction, Polymerase chain reaction - quantitative, Precipitation, Purification, Sequencing

Frequently asked questions - Extract-N-Amp™ Tissue PCR Kit

Can the isolated DNA be used in applications other than PCR such as restriction digestion or Southern blots? Can the PCR product amplified with the Extract-N-Amp Tissue PCR Kit be used for other down...
Keywords: Amplification, Digestions, Polymerase chain reaction, Polymerase chain reaction - quantitative, Sequencing

Sample Purification & Quality Assessment - PCR Technologies Guide

The availability of simple methods for purification of DNA and RNA has greatly facilitated the analysis and characterization of the genome and gene expression. There is a demand to isolate DNA and RN...
Keywords: AGE, Absorption, Amplification, Bio-Analytical, Buffers, Capillary electrophoresis, Cell disruption, Centrifugation, Chromatin immunoprecipitation, DNA purification, Degradations, Detection methods, Detergents, Digestions, Electrophoresis, Extinction coefficient, Filtration, Gel electrophoresis, Gene expression, Indicators, Lab-on-a-chip, Molecular biology, Nucleic acid hybridization, Nucleic acid purification, Polymerase chain reaction, Polymerase chain reaction - quantitative, Precipitation, Purification, RNA purification, Separation, Spectroscopy, Transcription, Ultraviolet-Visible spectroscopy

Protocols

Extract-N-Amp™ FFPE Tissue PCR Protocol Procedure

The following procedure is a modification of the Extract-N-Amp™ Tissue PCR protocol. Other DNA starting samples (PDF) may also be used. Download the full REDExtract-N-Amp™ Tissue PCR Kit Protocol (PD...
Keywords: AGE, Amplification, Evaporation, Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction, Sequencing

REDExtract-N-Amp™ Tissue PCR Kit Protocol

The REDExtract-N-Amp Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva. Brief...
Keywords: Amplification, Diagnostic, Evaporation, Gas chromatography, Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction, Precipitation, Purification, Sequencing

Related Content

Automated Protocols for Life Science Research

Scientists at Sigma-Aldrich® have validated all of the automated methods available below. Automation protocols are also provided as instruction for each of the methods. These protocols supply informa...
Keywords: Amplification, Polymerase chain reaction, Polymerase chain reaction - quantitative

Genomic DNA Extraction and Amplification Procedure Using REDExtract-N-Amp™ Tissue PCR Kit [VIDEO]

The REDExtract-N-Amp Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva. The a...
Keywords: Amplification, Digestions, Polymerase chain reaction, Sequencing

PCR Selection Guide

Sigma-Aldrich offers a wide variety of PCR reagents to meet any experimental needs. Our range of polymerases is customized to meet your End-Point PCR, qPCR, or RT-PCR needs. Our products vary from ro...
Keywords: Polymerase chain reaction, Polymerase chain reaction - quantitative

Peer-Reviewed Papers
15

References

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XNAT2 Extract-N-Amp Tissue PCR Kit, sufficient for 100 extractions, sufficient for 100 amplifications
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R4775 REDExtract-N-Amp PCR ReadyMix, Amplifications to support Extract-N-Amp Plant and Extract-N-Amp Tissue
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XNATG SYBR® Green Extract-N-Amp Tissue PCR Kit, sufficient for 100 extractions, sufficient for 100 amplifications
T3073 Tissue Preparation Solution, 30 mL sufficient for 1,000 extractions
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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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