• USA Home
  • XNATS - REDExtract-N-Amp Tissue PCR Kit

EMAIL THIS PAGE TO A FRIEND
XNATS Sigma

REDExtract-N-Amp Tissue PCR Kit

sufficient for 10 extractions, sufficient for 10 amplifications

Purchase

Properties

Related Categories Cell Biology, General Stem Cell Biology, Genomic DNA Amplification, Molecular Biology, Nucleic Acid Amplification,
usage   sufficient for 10 amplifications
  sufficient for 10 extractions
shipped in   wet ice
storage temp.   −20°C

Description

Components

Kits contain Tissue Preparation Solution, Extraction Solution, and REDExtract-N-Amp PCR Reaction Mix.

Features and Benefits

• Perfect for quick genomic DNA isolation for genotyping
• Single-step extraction of genomic DNA for PCR in 15 minutes
• PCR ReadyMix, specially formulated for amplification directly from extract
• Hot Start antibody for highly specific PCR amplification of genomic DNA
• Extract stable at 4 oC for at least 6 months

Other Notes

The REDExtract-N-Amp Tissue PCR Kits provide all the reagents necessary to rapidly extract DNA from a wide variety of cells and tissues and amplify targets of interest by PCR. A novel extraction method eliminates the need for long enzymatic digestions or homogenization. The kit also includes a specially formulated hot start PCR reaction mix for amplification directly from the extract. The REDExtract-N-Amp PCR ReadyMix contains an inert dye that acts as a tracking dye and allows for convenient loading of PCR reactions onto agarose gels for analysis.

For additional information, please see www.sigma-aldrich.com/extract-n-amp.

Legal Information

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Application

REDExtract-N-Amp tissue PCR kit has been for:
• DNA extraction
• genotyping
• polymerase chain reaction (PCR)

Principle

The kit comes with validated protocols to extract and amplify genomic DNA from mouse-tails, hair, animal tissue, saliva, and buccal swabs. In a typical procedure, genomic DNA is extracted from a sample that has been incubated in the tissue preparation solution and extraction solution for 10 minutes at room temperature. The sample is heated to 95 °C for 3 minutes and then mixed with a third solution to neutralize inhibitory substances prior to PCR. A portion of the DNA extract is then added to a PCR reaction containing primers and REDExtract-N-Amp PCR ReadyMix, included in the kit.

Price and Availability

Note: This product number has been replaced by the identical product XNAT - REDExtract-N-Amp™ Tissue PCR Kit.

All labs need water
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
Protocols & Articles

Articles

Extract-N-Amp™ Tissue Feature Article By: Scott Weber and Derek Douglas

Introduction Materials and Methods Results and Discussion Conclusions Acknowledgements References Ordering Information
Scott Weber and Derek Douglas
Sigma-Aldrich Corporation, St. Louis, MO, USA
Keywords: Amplification, Digestions, Polymerase chain reaction, Polymerase chain reaction - quantitative, Precipitation, Purification, Sequencing

Protocols

Extract-N-Amp™ FFPE Tissue PCR Protocol Procedure

The following procedure is a modification of the Extract-N-Amp™ Tissue PCR protocol. Other DNA starting samples (PDF) may also be used. Download the full REDExtract-N-Amp™ Tissue PCR Kit Protocol (PD...
Keywords: AGE, Amplification, Evaporation, Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction, Sequencing

REDExtract-N-Amp™ Tissue PCR Kit Protocol

The REDExtract-N-Amp Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva. Brief...
Keywords: Amplification, Diagnostic, Evaporation, Gas chromatography, Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction, Precipitation, Purification, Sequencing

Related Content

Genomic DNA Extraction and Amplification Procedure Using REDExtract-N-Amp™ Tissue PCR Kit [VIDEO]

The REDExtract-N-Amp Tissue PCR Kit contains all the reagents needed to rapidly extract and amplify genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva. The a...
Keywords: Amplification, Digestions, Polymerase chain reaction, Sequencing

PCR Selection Guide

Sigma-Aldrich offers a wide variety of PCR reagents to meet any experimental needs. Our range of polymerases is customized to meet your End-Point PCR, qPCR, or RT-PCR needs. Our products vary from ro...
Keywords: Polymerase chain reaction, Polymerase chain reaction - quantitative

Peer-Reviewed Papers
15

References

Related Products

application

Product #

Description

Add to Cart

N3910 Neutralization Solution B

related product

Product #

Description

Add to Cart

XNAT2 Extract-N-Amp Tissue PCR Kit, sufficient for 100 extractions, sufficient for 100 amplifications
XNAT2R Extract-N-Amp Tissue PCR Kit, sufficient for 1000 extractions, sufficient for 1000 amplifications
XNAT REDExtract-N-Amp Tissue PCR Kit
R4775 REDExtract-N-Amp PCR ReadyMix, Amplifications to support Extract-N-Amp Plant and Extract-N-Amp Tissue
E3004 Extract-N-Amp PCR ReadyMix, Amplifications to support Extract-N-Amp Plant and Extract-N-Amp Tissue
E8155 Extract-N-Amp PCR Diluent
E7526 Extraction Solution, Molecular Biology
S4320 SYBR® Green Extract-N-Amp PCR ReadyMix, Amplifications to support Extract-N-Amp Plant and Extract-N-Amp Tissue
XNATG SYBR® Green Extract-N-Amp Tissue PCR Kit, sufficient for 100 extractions, sufficient for 100 amplifications
T3073 Tissue Preparation Solution, 30 mL sufficient for 1,000 extractions
E0781 Extract-N-Amp PCR ReadyMix with qPCR Reference dye

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?