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Y0626 Sigma

Yeast Nitrogen Base Without Amino Acids

Yeast classification medium

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Description

Components

Component, amount (μg/L unless indicated)

Nitrogen Sources:
Ammonium sulfate, 5.0 g/L

Vitamins:
Biotin, 2.0
Calcium pantothenate, 400
Folic acid, 2.0
Inositol, 2.0 mg/L
Nicotinic acid, 400
p-Aminobenzoic acid, 200
Pyridoxine HCl, 400
Riboflavin, 200
Thiamine HCL, 400

Trace Elements:
Boric acid, 500
Copper sulfate, 40
Potassium iodide, 100
Ferric chloride, 200
Manganese sulfate, 400
Sodium molybdate, 200
Zinc sulfate, 400

Salts:
Potassium phosphate monobasic, 1.0 g/L
Magnesium sulfate, 0.5 g/L
Sodium chloride, 0.1 g/L
Calcium chloride, 0.1 g/L

Application

Suitable for use in classifying yeasts based on amino acid and carbon requirements. Addition of amino acids and carbon source (usually glucose) may be required.

General description

Yeast Nitrogen Base is a highly-referenced growth medium used for the cultivation of yeast. This nutrient-rich microbial broth contains nitrogen, vitamins, trace elements and salts.

Reconstitution

Make a 10x concentrated stock solution by stirring to suspend 6.8g yeast nitrogen base powder , 5 g glucose, and 5-10 mg of appropriate amino acid in 100 mL water. Warm if necessary to aid solubilization. Filter sterilize and store at 2-8°C.

Dilute the 10x concentrated stock to a 1x working solution by adding 100mL concentrated stock to 900mL sterile water.

Price and Availability


Amplified Detection
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Culture Method for Quality Control of Beer

Traditional methods are based morphology, staining methods, enzyme reactions (metabolism) and diverse media. One of the first steps is the filtration of the beer followed by an incubation of the filt...
Jvo Siegrist
Microbiology Focus Edition 1.4
Keywords: Fermentation, Filtration, Magnetic resonance spectroscopy, Metabolism, Microbiology

EZ-Product Family for Bioburden Testing

The EZ family of products has been designed to optimize and streamline the bioburden workflow. The family includes:
Keywords: Filtration, Separation

Introduction to Microbial Media

Microbiological studies require the availability of different species of bacteria, yeast or viruses in the laboratory. Large scale processes such as fermentation, protein and vaccine production requi...
Keywords: Antibiotics, Cellular processes, Cloning, Culture media, Fermentation, Gene expression, Growth factors, Indicators, Molecular biology, Nutrition, Sterilizations, Vitamins, transformation

Introduction to Yeast Media

Yeasts are eukaryotic microorganisms whose genomes have been comprehensively studied and some have been sequenced. They are relatively easy to grow under laboratory conditions. Moreover, despite thei...
Keywords: Culture media, Fermentation, Gene expression, Indicators, Molecular biology, Sterilizations, Transcription, Vitamins, transformation

Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the environment, microbes have adapted to the habitats most suitable for thei...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

Recombinant Protein Expression in Saccharomyces cerevisiae

Expressing recombinant proteins in yeast has many advantages, simple protocols, use of basic reagents, appropriate processing of eukaryotic proteins, and scalability. Sigma® Life Science is an essent...
Keywords: Cell disruption, Gene expression, transformation

Yeast Drop Out Bulletin

Catalog Number Y1501: without uracil Catalog Number Y1751: without histidine Catalog Number Y1896: without lysine Catalog Number Y1376: without leucine Catalog Number Y1876: without tryptophan Catalo...
Keywords: Filtration, transformation

Protocols

Yeast Growth Protocols

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells. Moreover, replica plating and mutant isolation of yeast cells can be done with relativ...
Keywords: Culture media, Detergents, Genetic, transformation

Peer-Reviewed Papers
15

References

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