Y1003 Sigma

2x YT medium

Liquid microbial growth medium

Synonym: 2xYT Medium



Related Categories Bacterial Culture Media, Broth / Media, Core Bioreagents, Life Science Reagents for Cloning, Microbial Media for Molecular Biology and Protein Expression,
Quality Level   GMP
grade   for molecular biology
sterility   sterile; 0.2 μm filtered
form   liquid
pH range   6.5 - 7.5
storage temp.   room temp
suitability   nonselective for Escherichia coli and Coliforms


Frequently Asked Questions

Frequently Asked Questions are available for this Product.


16 g/L Tryptone
10 g/L Yeast Extract
5.0 g/L NaCl


Suitable for non-selective cultivation of M13 bacteriophage, and E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.

Features and Benefits

2xYT liquid provides:
• Filter-sterilized, ready-to-use format
• Convenient package size
• Standard formulation

General description

2xYT is a standard microbial growth medium used for the cultivation of E. coli and M13 bacteriophage. This nutrient-rich microbial broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation.

Price and Availability

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option:
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
What is the difference among the 2x YT Media products?
2x YT Media is available in liquid (Y1003), powder (Y2377) and EZMix™ powder formats (Y2627).
Show more questions
Protocols & Articles


Introduction to Microbial Media

Microbiological studies require the availability of different species of bacteria, yeast or viruses in the laboratory. Large scale processes such as fermentation, protein and vaccine production requi...
Keywords: Antibiotics, Cellular processes, Cloning, Culture media, Fermentation, Gene expression, Growth factors, Indicators, Molecular biology, Nutrition, Sterilizations, Vitamins, transformation

Peer-Reviewed Papers


Related Products

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Add to Cart

Y2627 2xYT Medium, EZMix Powder microbial growth medium
Y2377 2x YT medium, Powder microbial growth medium

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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