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Y1250 Sigma

Yeast Nitrogen Base With Amino Acids

Non-selective yeast growth medium

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Properties

Related Categories Cloning and Expression, Core Bioreagents, HIS Select Supporting Products and Reagents, HIS-Select, Microbial Media,
grade   for molecular biology
sterility   non-sterile
form   powder
pH range   5.3 - 5.5 (0.67% solution)
storage temp.   room temp
suitability   nonselective for Candida
  nonselective for Pichia
  nonselective for Saccharomyces
  nonselective for Zygosaccharomyces

Description

Components

Component, amount (μg/L unless indicated)

Nitrogen Sources:
Ammonium sulfate, 5.0 g/L

Amino Acids:
L-histidine, 10 mg/L
DL-methionine, 20 mg/L
DL-tryptophan, 20 mg/L

Vitamins:
Biotin, 2.0
Calcium pantothenate, 400
Folic acid, 2.0
Inositol, 2.0 mg/L
Nicotinic acid, 400
p-Aminobenzoic acid, 200
Pyridoxine HCl, 400
Riboflavin, 200
Thiamine HCL, 400

Trace Elements:
Boric acid
Copper sulfate
Potassium iodide
Ferric chloride
Magnesium sulfate
Sodium molybdate
Zinc sulfate

Salts:
Potassium phosphate monobasic, 1.0 g/L
Magnesium sulfate, 0.5 g/L
Sodium chloride, 0.1 g/L
Calcium chloride, 0.1 g/L

General description

Yeast Nitrogen Base is a highly-referenced growth medium used for the cultivation of yeast. This nutrient-rich microbial broth contains amino acids, nitrogen, vitamins, trace elements and salts.

Packaging

250 g in poly bottle

Reconstitution

Make a 10x concentrated stock solution by stirring to suspend 6.7g yeast nitrogen base powder and 5 g glucose in 100 mL water. Warm if necessary to aid solubilization. Filter sterilize and store at 2-8°C.

Dilute the 10x concentrated stock to a 1x working solution by adding 100mL concentrated stock to 900mL sterile water.

Price and Availability


Amplified Detection
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Introduction to Microbial Media

Microbiological studies require the availability of different species of bacteria, yeast or viruses in the laboratory. Large scale processes such as fermentation, protein and vaccine production requi...
Keywords: Antibiotics, Cellular processes, Cloning, Culture media, Fermentation, Gene expression, Growth factors, Indicators, Molecular biology, Nutrition, Sterilizations, Vitamins, transformation

Introduction to Yeast Media

Yeasts are eukaryotic microorganisms whose genomes have been comprehensively studied and some have been sequenced. They are relatively easy to grow under laboratory conditions. Moreover, despite thei...
Keywords: Culture media, Fermentation, Gene expression, Indicators, Molecular biology, Sterilizations, Transcription, Vitamins, transformation

Recombinant Protein Expression in Saccharomyces cerevisiae

Expressing recombinant proteins in yeast has many advantages, simple protocols, use of basic reagents, appropriate processing of eukaryotic proteins, and scalability. Sigma® Life Science is an essent...
Keywords: Cell disruption, Gene expression, transformation

Protocols

Yeast Growth Protocols

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells. Moreover, replica plating and mutant isolation of yeast cells can be done with relativ...
Keywords: Culture media, Detergents, Genetic, transformation

Peer-Reviewed Papers
15

References

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Technical Service:

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