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Y1251 Sigma

Yeast Nitrogen Base Without Amino Acids and Ammonium Sulfate

for microbiology

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Description

Components

Ingredients
Biotin, 2 μg/L
Calcium pantothenate, 400 μg/L
Folic acid, 2 μg/L
Niacin, 400 μg/L
p-Aminobenzoic acid, 200 μg/L
Pyridoxine HCl, 400 μg/L
Riboflavin, 200 μg/L
Thiamine HCl, 400 μg/L
Inositol, 2 mg/L
Boric acid, 500 μg/L
Copper sulfate, 40 μg/L
Potassium iodide, 100 μg/L
Ferric chloride, 200 μg/L
Manganese sulfate, 400 μg/L
Sodium molybdate, 200 μg/L
Zinc sulfate, 400 μg/L
Potassium phosphate monobasic, 1 g/L
Magnesium sulfate, 0.5 g/L
Sodium chloride, 0.1 g/L
Calcium chloride, 0.1 g/L

Application

Recommended for use in classifying yeasts based on carbon and nitrogen requirements. Contains all essential nutrients required for the growth of yeasts except amino acids, nitrogen and carbohydrate. Addition of nitrogen and carbon sources is required.

Packaging

1 kg in poly bottle

100 g in poly bottle

Reconstitution

Preparation instructions
1. Prepare a 10× stock solution by suspending 1.7 g of yeast nitrogen base without amino acids and ammonium sulfate in 100 mL of cold distilled water. Include desired nitrogen and carbon sources.
2. Warm if necessary to solubilize and sterilize by filtration.
Store 10× stock solution at 2-8 °C. To use, dilute 1:10 with sterile distilled water under aspetic conditions.

Price and Availability


Amplified Detection

Kit component only

Description

Product #

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KH2PO4 1 g/L SDS    
MgSO4 .5 g/L SDS    
NaCl .1 g/L SDS    
CaCl2 .1 g/L SDS    
Inositol 2 mg/L SDS    
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Culture Method for Quality Control of Beer

Traditional methods are based morphology, staining methods, enzyme reactions (metabolism) and diverse media. One of the first steps is the filtration of the beer followed by an incubation of the filt...
Jvo Siegrist
Microbiology Focus Edition 1.4
Keywords: Fermentation, Filtration, Magnetic resonance spectroscopy, Metabolism, Microbiology

Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the environment, microbes have adapted to the habitats most suitable for thei...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

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