|Related Categories||All Liquid Media/Broths, Escherichia coli & Coliforms, HIS Select Supporting Products and Reagents, HIS-Select, Media,|
|grade||for molecular biology|
|pH range||6.8 - 7.2 (3.1% solution)|
|storage temp.||room temp|
|suitability||nonselective for Escherichia coli and Coliforms|
16 g/L Tryptone
10 g/L Yeast Extract
5 g/L NaCl
Suitable for non-selective cultivation of M13 bacteriophage, and E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.
2xYT powder provides:
• Standard formulation
• An inexpecnsive alternative to liquid
2xYT is a standard microbial growth medium used for the cultivation of E. coli and M13 bacteriophage. This nutrient-rich microbial broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation.
Add 31 g powder in 1L water. Heat to boiling while stirring to dissolve powder. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).
Microbiological studies require the availability of different species of bacteria, yeast or viruses in the laboratory. Large scale processes such as fermentation, protein and vaccine production requi...
Keywords: Antibiotics, Cellular processes, Cloning, Fermentation, Gene expression, Growth factors, Indicators, Molecular biology, Nutrition, Sterilizations, Vitamins, transformation
From our library of Protocols, Sigma-Aldrich presents Microbial Growth Protocols
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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