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  • Y4645 - Anti-phospho-YAP1 (pTyr357) antibody produced in rabbit

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Y4645 Sigma

Anti-phospho-YAP1 (pTyr357) antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym: Anti-YAP2, Anti-YAP65, Anti-YAP, Anti-Yes-associated protein 1, 65kDa

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Antibodies to Phosphoproteins at Phosphotyrosine (pTyr),
species reactivity   human
application(s)   indirect immunofluorescence: 5-10 μg/mL using HEK-293 cells co-transfected with human YAP1 and c-Abl
  western blot: 0.5-1 μg/mL using HEK-293T cells co-transfected with human YAP1 and human c-Abl
clone   polyclonal
concentration   ~1.5 mg/mL
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt ~65 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   chicken ... YAP1(396171)
human ... YAP1(10413)
mouse ... Yap1(22601)
biological source   rabbit
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 351-362 (pTyr357) located in the C-terminal region of human YAP1. The sequence is identical in chicken YAP1 and highly conserved in mouse YAP1 (single amino acid substitution).

General description

YAP1 is a WW domain containing protein that is known to associate with a wide range of transcription factors and functions as a transcriptional co-activator. It may also regulate p73 gene function in response to DNA damage. Alterations in YAP1 have been associated with tumor development
Anti-phospho-YAP1 pTyr357 antibody is specific for human phospho-YAP1 pTyr357. The product may react with chicken and mouse phospho-YAP1 pTyr357. In immunoblotting, staining of the phospho-YAP1 (pTyr357) band is specifically inhibited by the immunizing peptide; but no inhibition is observed with the unmodified peptide.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Application

Anti-phospho-YAP1 pTyr357 antibody is suitable for use in western blot (0.5-1 μg/mL ) and indirect immunofluorescence (5-10 μg/mL) using HEK-293T cells co-transfected with human YAP1 and human c-Abl.

Features and Benefits

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