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Fmoc Solid Phase Peptide Synthesis: A Practical Approach

  •  ISBN-10 0-19-963724-5

  •  ISBN-13 978-0-19-963724-9

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publication info   W.C. Chan and P.D. White, ed., Oxford University Press, 2000, 288 pp., soft cover
mfr. no.   Oxford University Press

Description

General description

This new volume covers essential procedures for production of linear peptides and more advanced techniques for preparing cyclic, side-chain modified, phospho- and glycopeptides. Many other methods have been included: convergent peptide synthesis; peptide-protein conjugation; chemoselective ligation; and chemoselective purification. The difficult preparation of cysteine and methionine-containing peptides is covered, as well as methods for overcoming aggregation during peptide chain assembly.

Table of Contents

List of Contributors
Abbreviations
1. Introduction--a retrospective viewpoint
2. Basic principles, Peter D. White and Weng C. Chan
3. Basic procedures, Weng C. Chan and Peter D. White
4. Preparation and handling of peptides containing methionine and cysteine, Fernando Albericio et al.
5. Difficult peptides, Martin Quibell and Tony Johnson
6. Synthesis of modified peptides, Sarah L. Mellor et al.
7. Phosphopeptide synthesis, Peter D. White
8. Glycopeptide synthesis, Jan Kihlberg
9. Convergent peptide synthesis, Kleomenis Barlos and Dimitrios Gatos
10. Methods of preparing peptide-carrier conjugates, Jan W. Driifhout and Peter Hoogerhout
11. Chemoselective and orthoggonal ligation techniques, James P. Tam and Y.-A. Lu
12. Purification of large peptides using chemoselective tags, Paolo Mascagni
13. Instrumentation for automated solid phase peptide synthesis, Linda E. Cammish and Steven A. Kates
14. Manual multiple synthesis methods, B. Dorner et al.
Appendices
Index

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