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Z340820 Sigma

Protein Expression: A Practical Approach

  •  ISBN-10 0-19-963623-0

  •  ISBN-13 978-0-19-963623-5

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publication info   B.D. Hames and S. J. Higgins, ed., Oxford University Press, 1999, 304 pp., soft cover
mfr. no.   Oxford University Press

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General description

Details of the expression of cloned DNA or RNA templates in all the major in vivo and in vitro systems are included such as cultured mammalian cells, the yeasts Saccharomyces cerevisiae and Pichia pastoris, baculovirus, Xenopus oocytes and prokaryotic cells. Cell-free systems of both eukaryotes and prokaryotes are described in addition to the prokaryotic systems that offer coupled transcription-translation.

Table of Contents

1. Protein expression in mammalian cells, Marlies Otter-Nilsson and Tommy Nilsson
2. Expression in Xenopus oocytes and cell-free extracts, Glenn M. Matthews
3. Expressing cloned genes in the yeasts Saccharomyces cerevisiae and Pichia pastoris, Mick F. Tuite, Jeff J. Clare, and Mike A. Romanos
4. Baculovirus expression systems, Claire L. Merrington, Linda A. King, and Robert D. Possee
5. Protein synthesis in eukaryotic cell-free systems, Mike J. Clemens and Ger J. M. Pruijn
6. Prokaryotic in vivo expression systems, Edward R. Appelbaum and Allan R. Shatzman
7. Cell-free coupled transcription-translation systems from Escherichia coli, Gisela Kramer, Wieslaw Kudlicki, and Boyd Hardesty
8. Monitoring protein expression, John Colyer

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