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Z359327 Sigma

Hybridomas and Monoclonal Antibodies

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publication info   C. Brinton, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 1994
mfr. no.   Cold Spring Harbor

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This video guide, produced at the University of Pittsburgh Hybridoma Center, demonstrates the technical steps required for the successful production of antibodies: immunization and bleeding of mice, fusion of spleen cells and myeloma cells with PEG and DMSO, HAT selection and screening off positive cultures and growth of hybridoma lines. The demonstrations are accompanied by audio commentary on the procedures involved and their theoretical basis. (32 minutes)

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