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  • Z364231 - Glycobiology: A Practical Approach

Z364231 Sigma

Glycobiology: A Practical Approach

  •  ISBN-10 0-19-963371-1

  •  ISBN-13 978-0-19-963371-5



publication info   D. Rickwood and B.D. Hames, ed., IRL Press at Oxford University Press, Oxford, England, 1993, 390 pp., soft cover
mfr. no.   Oxford University Press


General description

Describes the essential analytical techniques to study the structure and biosynthesis of glycoproteins.

Table of Contents

1. Isolation and characterization of glycoproteins
2. Compositional analysis of glycoproteins
3. Isolation and fractionation of N-linked oligosaccharides from glycoproteins
3a. Release of N-linked oligosaccharides and fractionation by gel filtration
3b. Fractionation of oligosaccharides by serial affinity chromatography with use of immobilized lectin columns
3c. Fractionation of glycopeptides and oligosaccharides from glycoproteins by HPLC
4. Structural characterization of oligosaccharides from glycoproteins
4a. Glycosidase treatment and other methods, including methylation analysis
4b. FAM-MS and ES-MS of glycoproteins
4c. Nuclear magnetic resonance spectroscopy of glycoprotein-derived carbohydrate chains
5. Structural characterization of N-glycans obtained from metabolically-radiolabelled glycoproteins
6. Structural characterization of mucin-type O-linked oligosaccharides
7. Characterization of nuclear and cytoplasmic glycoproteins
8. GPI membrane anchors

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