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Z373826 Sigma

Laboratory Guide to RNA

  •  ISBN-10 0-471-12536-9

  •  ISBN-13 978-0-471-12536-5



publication info   P. Krieg, ed., Wiley-Liss, 1996, 405 pp., hard cover
mfr. no.   Wiley


General description

This guide provides detailed accounts of experimental procedures ranging from the isolation, analysis, and quantification of RNA from a variety of sources to large and small scale synthesis of RNA. Protocols such as RNA blotting, nuclease protection, and in situ hybridization are covered. A solid reference on the analysis of gene expression at the RNA level.

Table of Contents

Partial table of contents:  RNA Isolation from Cells and Tissues (M. Mehra).  Isolation of RNA from Plant Tissue (T. Wilkins & L. Smart).  Analysis of Polysomal RNA (O. Meyuhas, et al.).  Primer-Extension Analysis of RNA (C. George & J. Kadonaga).  In Vitro Translation of Cellular and Synthetic RNA (A. Kaminski & N. Standart).  Quantitation of RNA Transcripts Using RT-PCR (F. Ferré, et al.).  Analysis of Messenger RNA by Differential Display (P. Liang).  A New Methods for Full-Length cDNA Cloning By PCR (A. Chenchik, et al.).  Whole-Mount In Situ Hybridization to Mouse Embryos (R. Conlon).  Analyzing Patterns of Alternative Splicing (C. Smith, et al.).  Index.

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