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Z374911 Sigma

PCR multiwell plates

384 well PCR plate, polypropylene, skirted, non-sterile, 50/cs

Synonym: 384 multiwell PCR plate, 384 well PCR microplate, 384 well PCR plate, 384 well microtiter plate

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Properties

Related Categories Labware, Molecular Biology Supplies, PCR (Polymerase Chain Reaction) Supplies, PCR Plates
material   colorless polypropylene plate
sterility   non-sterile
feature   plate format: 384 well standard
packaging   case of 50
mfr. no.   Sorenson, 39620
well volume   40 μL
well working volume   25 μL
suitability   suitable for (PCR, RT-PCR or DNA purification applications)
Featured Industry   Agriculture

Description

Features and Benefits

• Virgin polypropylene
• Fully autoclavable
• Certified DNase- and RNase-free
• Wells have thin walls for rapid temperature equilibration and reduced cycle time

General description

384 well PCR plates are skirted for compatibility with automation systems. Wells have raised rims to ensure contact with sealing film and reduce evaporation. Each well has a capacity of 40 μL and a working volume of 25 μL.

A rigid top plate (included) minimizes plate distortion, assures a dependable fit with the PCR thermal cycler, and allows for a leak-proof seal with micro-mats or cap strips. Each well has a maximum capacity of 200 μL.

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Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.
Protocols & Articles

Protocols

Amplification of Damaged DNA with Restorase DNA Polymerase (R1028)

Restorase® DNA Polymerase with 10x Reaction Buffer combines Sigma's Long and Accurate enzyme technology with a DNA repair enzyme resulting in a blend that facilitates repair and amplification of dama...
Keywords: AGE, Alkylations, Amplification, Applications, Buffers, Catalog, Catalysis, Degradations, Diagnostic, Electrophoresis, Gas chromatography, Gel electrophoresis, Instructions, Melting, Methods, Nucleic acid annealing, Nucleic acid denaturation, Nucleic acid hybridization, Polymerase chain reaction, Precipitation, Protocols, Sequences, Size-exclusion chromatography

Amplification of Genomic DNA using REDTaq DNA Polymerase

REDTaq DNA Polymerase is a convenient package that includes all the necessary components for a PCR reaction except primers, DNA template and water. The formulation has been optimized for amplificatio...
Keywords: AGE, Amplification, Applications, Buffers, Digestions, Electrophoresis, Evaporation, Formulations, Gel electrophoresis, Polymerase chain reaction, Purification, Sequencing, transformation

Antibody-Enzyme Mediated Hot Start PCR Protocol

During PCR assay preparation, nonspecific amplification can occur due to binding of PCR primers to nonspecific templates and from formation of primer dimers which result from using other primer molec...
Keywords: AGE, Amplification, Buffers, Electrophoresis, Enzyme activity, Gel electrophoresis, Nucleic acid denaturation, Polymerase chain reaction, Size-exclusion chromatography

Hot Start Taq Polymerase Protocol to Reduce Non-Specific Amplification

As PCR reactions sit at room temperature, during assay setup, nonspecific amplification can occur via:
Keywords: AGE, Amplification, Electrophoresis, Gel electrophoresis, Gene expression, Nucleic acid annealing, Polymerase chain reaction, Size-exclusion chromatography

Kicqstart SYBR qPCR | Universal SYBR Green qPCR Protocol

KiCqStart SYBR Green qPCR ReadyMix is a 2X concentrated, ready-to-use reaction cocktail that contains all components, except primers and template, for real-time quantitative PCR (qPCR). This unique c...
Keywords: Amplification, Gene expression, Molecular probes, Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction, Polymerase chain reaction - quantitative, Size-exclusion chromatography, Titrations

Long and Accurate PCR Amplification of DNA with RedAccuTaq D4812 Protocol

Reaction Optimization Reliable amplification of long DNA sequences requires: 1) effective denaturation of DNA template, 2) adequate extension times to produce large products and 3) protection of targ...

Multiplex qPCR Protocol

In the PCR Technologies Guide, the requisite components and quality control requirements for qPCR experiments were described in detail. With those in mind, the following is a protocol that can be use...
Keywords: Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction, Polymerase chain reaction - quantitative, Size-exclusion chromatography

MystiCq® MicroRNA® Quantitation System

Technology Overview Workflow Background Protocols miRNA isolation cDNA synthesis miRNA qPCR Advanced information Product selection guides Troubleshooting
Keywords: AGE, Amplification, Buffers, Cell disruption, Centrifugation, Degradations, Diagnostic, Digestions, Diseases, Electrophoresis, Gel electrophoresis, Gene expression, Homogenization, Microarray Analysis, Molecular biology, Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction, Polymerase chain reaction - quantitative, Precipitation, Purification, RNA purification, Sample preparations, Separation, Solvents, Titrations

PCR amplification of Bacterial DNA with low contamination using MTP™ Taq DNA Polymerase

MTP™ Taq DNA Polymerase is a recombinant thermostable enzyme from Thermus aquaticus expressed in E. coli and purified using a proprietary process to minimize levels of contaminating DNA. The enzyme h...
Keywords: AGE, Amplification, Applications, Electrophoresis, Evaporation, Gel electrophoresis, Gene expression, Methods, PAGE, Polymerase chain reaction, Sequences

Primer Concentration Optimization Protocol

Optimization of qPCR conditions is important for the development of a robust assay. Indications of poor optimization are a lack of reproducibility between replicates as well as inefficient and insens...
Keywords: Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction, Polymerase chain reaction - quantitative, Size-exclusion chromatography

Primer Optimization Using Temperature Gradient Protocol

One approach to assay optimization is to determine the optimum annealing temperature (Ta) of the primers by testing identical reactions containing a fixed primer concentration, across a range of anne...
Keywords: Amplification, Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction, Polymerase chain reaction - quantitative, Size-exclusion chromatography

Reverse Transcription Protocol (One-step Probe Detection)

In some cases it is preferable to measure the target transcript directly, without preparing cDNA from the entire RNA sample. Such situations may include measurements on highly degraded RNA or when th...
Keywords: Nucleic acid denaturation, Polymerase chain reaction, Polymerase chain reaction - quantitative, Size-exclusion chromatography, Transcription

Reverse Transcription Protocol (One-step SYBR Green I Dye Detection)

In the example given below, the primer concentrations can be adjusted according to the results of optimization procedures (see Primer Concentration Optimization, Primer Optimization Using Temperature...
Keywords: Nucleic acid denaturation, Polymerase chain reaction, Polymerase chain reaction - quantitative, Size-exclusion chromatography

SPUD Assay for Detection of Assay Inhibitors Protocol

The SPUD assay is one option for identification of inhibitors that may be present in RNA or DNA samples. The assay is particularly useful when a large number of samples are to be analyzed or when tar...
Keywords: Amplification, Nucleic acid denaturation, Polymerase chain reaction, Polymerase chain reaction - quantitative, Size-exclusion chromatography

SYBR® Green I Dye Quantitative PCR Protocol

Although quantitative PCR uses the same basic concept as traditional PCR, the reactions differ in that the amplicons are generally smaller and are detected indirectly using an additional dye or label...
Keywords: Gene expression, Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction, Polymerase chain reaction - quantitative, Size-exclusion chromatography

Standard PCR protocol

Taq DNA Polymerase is a thermostable enzyme derived from the thermophilic bacterium Thermus aquaticus. The enzyme is in a recombinant form, expressed in E. coli. It is able to withstand repeated heat...
Keywords: AGE, Amplification, Applications, Electrophoresis, Evaporation, Gel electrophoresis, PAGE, Polymerase chain reaction, Sequencing

Standard Reverse Transcription Protocol (Two-step)

Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.
Keywords: Amplification, Polymerase chain reaction, Polymerase chain reaction - quantitative, Transcription

The 3'/5' Assay for Analysis of RNA Integrity Protocol

The 3’/5’ integrity assay is a potential first step in the identification of RNA degradation. The assay is particularly useful when a large number of samples are to be analyzed or when the degradatio...
Keywords: Amplification, Degradations, Nucleic acid denaturation, Polymerase chain reaction, Polymerase chain reaction - quantitative, Size-exclusion chromatography, Transcription

Universal SYBR Green Quantitative PCR Protocol

Technology Overview Assay Considerations Methods of Quantification Equipment & Supplies PCR Mix Selection Guide Protocol Troubleshooting Materials References
Keywords: AGE, Amplification, Buffers, Degradations, Electrophoresis, Enzyme activity, Gas chromatography, Gel electrophoresis, Gene expression, Genetic, Melting, Microarray Analysis, Nucleic acid annealing, Nucleic acid denaturation, Nucleic acid hybridization, Polymerase chain reaction, Polymerase chain reaction - quantitative, Polymorphisms, Purification, Sample preparations, Size-exclusion chromatography, Solvents, Titrations

dNTP Mediated Hot Start PCR Protocol

Hot Start dNTPs are modified with a thermolabile protecting group at the 3’ terminus. The presence of this modification blocks nucleotide incorporation by DNA polymerase until the nucleotide protecti...
Keywords: AGE, Amplification, Buffers, Centrifugation, Degradations, Electrophoresis, Gel electrophoresis, Nucleic acid denaturation, Polymerase chain reaction, Purification, Size-exclusion chromatography, Transcription

qPCR Efficiency Determination Protocol

Once an assay has been optimized, it is important to verify the reaction efficiency. This information is important when reporting and comparing assays. In this example protocol, the assay efficiency ...
Keywords: Amplification, Gene expression, Nucleic acid denaturation, Polymerase chain reaction, Polymerase chain reaction - quantitative, Size-exclusion chromatography, Transcription

qPCR Gene Expression/Copy Number Analysis Using SYBR Green I Dye Detection Protocol

Measuring a target quantity relative to one or more stable reference genes using SYBR Green I dye detection is a common application of qPCR. Below is a standard protocol that can be adapted to specif...
Keywords: Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction, Polymerase chain reaction - quantitative, Size-exclusion chromatography, Transcription

qPCR Gene Expression/Copy Number/SNP Analysis Using Probe Detection Protocol

The most common application for qPCR is the measurement of a gene transcript or copy number quantity relative to one or more reference genes using probe detection. The reactions may be designed such ...
Keywords: Nucleic acid denaturation, Polymerase chain reaction, Polymerase chain reaction - quantitative, Size-exclusion chromatography, Transcription

qPCR Reference Gene Selection Protocol

Analysis of gene expression data requires a stable reference or loading control. This reference is usually one or more reference genes. Suitable reference genes are those which are unaffected by diff...
Keywords: Gene expression, Nucleic acid denaturation, Polymerase chain reaction, Polymerase chain reaction - quantitative, Size-exclusion chromatography, Transcription

qPCR Using a Single Detection Probe Protocol

In the PCR Technologies Guide, the requisite components and quality control requirements for qPCR experiments were described in detail. With those in mind, the following is a protocol that can be use...
Keywords: Nucleic acid annealing, Nucleic acid denaturation, Polymerase chain reaction, Polymerase chain reaction - quantitative, Size-exclusion chromatography

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