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Bioluminescence Methods and Protocols: Methods in Molecular Biology, Vol. 102

  •  ISBN-10 0-89603-520-4

  •  ISBN-13 978-0-89603-520-1

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publication info   R.A. LaRossa, ed., Humana Press, US, 1998, 320 pp., hard cover
mfr. no.   Humana

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General description

A collection of methods for using luciferase and green fluorescent protein in analytical biochemistry, molecular toxicology, and cell-based assays. The contributors are those who have developed and perfected their methods. The wide variety of illustrative applications ranges from specific molecular titer to microbial detection and analysis. Other topics include the manipulation of the lux, luc, and gfp genes and construction of recombinant organisms. Cutting-edge techniques, detailed instructions, and diverse examples should make this book a standard reference.

Table of Contents

Part I. The Basics. Improvements in the Application of Firefly Luciferase Assays. Visualization of Bioluminescence, Vollmer. Microscopic Imagery of Mammalian Cells Expressing an Enhanced Green Fluorescent Protein Gene. Part II. Analytical Biochemistry. Luminometric Measurement of Malate and Glucose-6-Phosphate in Mammalian Tissue. Bioluminescent Assay of the Guanylates. Bioluminescent Assay of the Adenylate Energy Charge. Part III. Molecular Biology Tools. Photorhabdus luminescent luxCDABE Promoter Probe Vectors. Insertion of Promoter Region: luxCDABE Fusions Into the Escherichia Coli Chromosome. Probing for Promoters With Luciferase-Transposons. Cryopreservation and Reawakening. Luciferase Renaturation Assays of Chaperones and Chaperone Antagonists, Part IV. Cell-Based Assays. Genotoxic Sensors, r. Stress Detection Using Bioluminescent Reporters of the Heat -Shock Response. Real-Time Reporter of Protein Synthesis Inhibition, Luminescence-Based Cell Viability Testing, Phagocyte Chemiluminescence. Detection of Oxidants Using lux Fusions to Oxidative Stress Promoters. Part V. Environmental Applications. Luciferase-Based Measurement of Water Contaminants, Bioluminescence-Based Metal Detectors, Luminescence Facilitated Detection of Bioavailable Mercury in Natural Waters, A Panel of Stress-Responsive Luminous Bacteria for Monitoring Wastewater Toxicity. Organic Contaminant Detection and Biodegration Characteristics, Detection of Firefly Luciferase-Tagged Bacteria in Environmental Samples, Monitoring of GFP-Tagged Bacteria Cells,

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