Real-Time PCR
M. Dorak, Garland Science, 2006, 333 pp., soft cover
DOWNLOAD MSDS (PDF)-
ISBN-13 978-0415377348
Properties
| Related Categories | Books and Software, Labware, Molecular Biology Books More... |
| publication info | M. Dorak, Garland Science, 2006, 333 pp., soft cover |
Description
General description
With a variety of detection chemistries, an increasing number of platforms, multiple choices for analytical methods and the jargon emerging along with these developments, real-time PCR is facing the risk of becoming an intimidating method, especially for beginners. Real-time PCR provides the basics, explains how they are exploited to run a real-time PCR assay, how the assays are run and where these assays are informative in real life. It addresses the most practical aspects of the techniques with the emphasis on ′how to do it in the laboratory′.
Table of Contents
1. An Introduction to Real-time PCR
2. Data Analysis and Reporting
3. Relative Quantification
4. Normalisation
5. High-Throughput Primer and Probe Design
6. Quantitative Analysis of Ocular Gene Expression
7. Quantitative Gene Expression by Real-time PCR
8. Real-time PCR Using SYBR Green
9. High-Resolution Melting Analysis for Scanning and Genotyping
10. Quantitative Analyses of DNA Methylation
11. Mitochondrial DNA Analysis
12. Real-time Immuno-PCR
13. Clinical Microbiology
14. Clinical Virology
15. Solid Organ Transplant Monitoring
16. Real-time PCR Applications in Haematology
17. Prenatal Diagnosis in Clinical Genetics for Monogenic Diseases Caused by Single Nucleotide Changes
Price and Availability
Customers Also Viewed
100 ×, solution
One step SYBR® Green RT-qPCR with MMLV & hot-start Taq DNA Polymerase
Related Papers
Technical Service:
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Bulk Ordering & Pricing:
Need larger quantities for your development, manufacturing or research applications?
