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47868 Supelco

D-Biotin

analytical standard

Synonym: D-Biotin, Bios II, Biotin, Coenzyme R, Vitamin B7, Vitamin H

  • CAS Number 58-85-5

  • Empirical Formula (Hill Notation) C10H16N2O3S

  • Molecular Weight 244.31

  •  Beilstein Registry Number 86838

  •  EC Number 200-399-3

  •  MDL number MFCD00005541

  •  PubChem Substance ID 24871542

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Properties

Related Categories Additional Standards, Analytical Standards, Analytical/Chromatography, Application Areas and Market Segments, Biochemicals and Reagents,
grade   analytical standard
InChI Key   YBJHBAHKTGYVGT-ZKWXMUAHSA-N
form   neat
feature   standard type water soluble vitamin
packaging   ampule of 100 mg
application(s)   HPLC: suitable
  gas chromatography (GC): suitable
mp   231-233 °C(lit.)
format   neat
storage temp.   2-8°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Application

Refer to the product′s Certificate of Analysis for more information on a suitable instrument technique. Contact Technical Service for further support.

Price and Availability

Suggested Laboratory Gloves


Laboratory GlovesThis substance has been tested against several types of hand protection for CE compliance. Click below to find the recommended gloves for handling this product.




All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
XJ9088200

Documents

Certificate of Analysis

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
Is Product 47868, D-Biotin, suitable for cell culture?
Product 47868 D-Biotin, is not cell culture tested, and you should not consider it for cell culture use.  The Supelco biotin is a chemical standard to be used in chromatographic methods. For cell culture, consider either the animal cell culture tested biotin (Product No. B4639), or the plant cell culture tested biotin (Product No. B3399).
How do I solubilize Product 47868, D-Biotin?
Per the Merck Index, 13th ed, biotin is readily soluble in hot water or dilute alkali; it is insoluble in other common organic solvents.  In our labs, the basic solutions may be Trizma base, 2M ammonium hydroxide, or 1N sodium hydroxide.  You can put the biotin into water, and titrate it with base until it goes into solution.
What column do you recommend for the analysis of water soluble vitamins, including Biotin?
The Discovery C18 and Ascentis RP-Amide HPLC columns have been used for the separation of ten water soluble vitamins: Ascorbic acid, Biotin, Cyanocobalamin, Folic acid, Niacin, Niacinamide, Pantothenic acid, Pyridoxine, Riboflavin, & Thiamine. Contact Supelco Technical Service for details -- http://techservice@sial.com
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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Protocols & Articles

Articles

HPLC Analysis of B-vitamins on Ascentis® Express RP-Amide using UV detection

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of B-vitamins on Ascentis® Express RP-Amide using UV detection
Keywords: Anticonvulsants, Chromatography, Pharmaceutical, Vitamins

HPLC Analysis of Biotin Enantiomers on Astec® CHIROBIOTIC® T

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Biotin Enantiomers on Astec® CHIROBIOTIC® T
Keywords: Chromatography, High performance liquid chromatography, Vitamins

HPLC Analysis of Water Soluble B Vitamins on Ascentis® Express RP-Amide

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Water Soluble B Vitamins on Ascentis® Express RP-Amide
Keywords: Anticonvulsants, Chromatography, High performance liquid chromatography, Pharmaceutical, Vitamins

HPLC Analysis of Water-Soluble B-Vitamins on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Water-Soluble B-Vitamins on Ascentis® Express C18
Keywords: Anticonvulsants, Chromatography, High performance liquid chromatography, Pharmaceutical, Vitamins

HPLC Analysis of Water-Soluble B-Vitamins on Ascentis® Express F5

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Water-Soluble B-Vitamins on Ascentis® Express F5
Keywords: Anticonvulsants, Chromatography, High performance liquid chromatography, Pharmaceutical, Vitamins

UHPLC Analysis of B-vitamins on Titan™ C18 using UV detection

From our library of Articles, Sigma-Aldrich presents UHPLC Analysis of B-vitamins on Titan™ C18 using UV detection
Keywords: Anticonvulsants, Chromatography, Pharmaceutical, Vitamins

Peer-Reviewed Papers
15

References

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