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50619-U Supelco

Ascentis® Express F5, 5 Micron HPLC Column

5 μm particle size, L × I.D. 7.5 cm × 3.0 mm

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Properties

Related Categories Analytical/Chromatography, Ascentis Express Columns for Fast HPLC, Ascentis Express F5, 5 μm HPLC Columns, F5, 5 μm Columns, HPLC,
feature   endcapped
limit   60 °C temp. range
L × I.D.   7.5 cm × 3.0 mm
impurities   <5 ppm metals
matrix   Fused-Core particle platform
particle size   5 μm
pore size   90 Å pore size
operating pH   1-9
suitability   suitable for L43 per USP
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Description

General description

Ascentis® Express 5 μm F5 is a high-speed, high-performance liquid chromatography column based on the highly efficient Fused-Core® particle design. The bonded, endcapped, dimethylpentafluorophenyl-propylsilane stationary phase of Ascentis Express 5 μm F5 provides a stable, reversed-phase packing with electron-deficient phenyl rings due to the presence of electronegative fluorines. In addition to forming π-π and mildly steric interactions, F5 phases also retain compounds by polar interactions. As a result of having both polar and non-polar character, F5 phases can show dual-mode retention behavior, sometimes producing a “U-shaped” retention as a function of acetonitrile content of the mobile phase, with retention increasing at both low and high concentrations of ACN (reversed-phase and HILIC retention modes). Ascentis Express 5 μm F5 can be used for basic, acidic, or neutral compounds with alternate selectivity from C18.

Legal Information

Ascentis is a registered trademark of Sigma-Aldrich Co. LLC

Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

Price and Availability

NEW! Ascentis® Express 5 µm
HPLC Columns

New Ascentis Express 5 µm HPLC Columns!Ascentis Express columns, based on 5 µm Fused-Core® particles, offer better performance, faster and more efficient separations, compared to columns based on 3 µm and 5 µm totally porous particles, with no backpressure concerns!


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