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  • 53825-U - Ascentis® Express C18, 2.7 Micron HPLC Column

53825-U Supelco

Ascentis® Express C18, 2.7 Micron HPLC Column

2.7 μm particle size, L × I.D. 15 cm × 2.1 mm

  •  eCl@ss 32110501



Related Categories Analytical/Chromatography, Ascentis Express C18, 2.7μm HPLC Columns, Ascentis Express Columns for Fast HPLC, C18, 2.7μm Columns, HPLC,
feature   endcapped
limit   60 °C temp. range
application(s)   HPLC: suitable
L × I.D.   15 cm × 2.1 mm
impurities   <5 ppm metals
matrix   Fused-Core particle platform
matrix active group   C18 (octadecyl) phase
particle size   2.7 μm
pore size   90 Å
operating pH   2 - 9
suitability   suitable for L1 per USP
mode of chromatography   Reversed phase


Frequently Asked Questions

Frequently Asked Questions are available for this Product.

General description

Ascentis Express HPLC columns, through the use of Fused-Core® particle technology, can provide you with both the high speed and high efficiencies of sub-2 μm particles while maintaining lower backpressures. The combination of high efficiency and low backpressure benefits UPLC® (or other ultra high pressure system) users, as well as conventional HPLC users.
Visit the Ascentis Express home page for more information on this new column technology.

Legal Information

Ascentis is a registered trademark of Sigma-Aldrich Co. LLC

Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

UPLC is a registered trademark of Waters

Price and Availability

Safety & Documentation

Safety Information

GHS07  GHS07
Signal word 
Hazard statements 
Precautionary statements 
WGK Germany 
Protocols & Articles


Analysis of Bisphenol A in Broth-Based Soup Matrices

Introduction The use of bisphenol A (BPA)-based plastics in food and beverage containers has been a point of controversy for the past decade. Although these clear, tough plastics have been commercial...
K. G. Espenschied, Jennifer Claus, Michael Ye
Reporter US Volume 33.3
Keywords: Cancer, Evaporation, High performance liquid chromatography, Obesity, Size-exclusion chromatography, Solid phase extractions, Solvents

Ascentis Express UHPLC Columns with 2.0 μm Fused-Core Particles

Column Performance The new Supelco Ascentis® Express column line has been designed specifically for UHPLC instruments with 2.0 µm Fused-Core® silica, as diagrammed in Figure 1. Ascentis Express 2.0 µ...
Richard A. Henry, Gaurang Parmar
Reporter US, Volume 32.2
Keywords: High performance liquid chromatography, Mass spectrometry, Pharmaceutical, Separation

Better Tools for Fast and Convenient Patulin Analysis

By: Eva Katharina Richter, Christine Dumas, Sigma-Aldrich Analytical Standards and NEW SupelMIP® SPE Cartridges
Keywords: Gastrointestinal, High performance liquid chromatography, Phase transitions, Purification, Sample preparations, Separation, Solid phase extractions, Solvents

Derivatization and Separation of Aliphatic Amines

Hugh Cramer, Associate Scientist Shyam Verma, Market Segment Manager Michael Kiselewsky, Product Manager
AnalytiX, Volume 2, 2011
Keywords: Absorption, Derivatizations, High performance liquid chromatography, Separation

Rapid Screening of Pharmaceutical Compounds | Fast HPLC

HPLC is critical to the discovery, development and eventual commercialization of pharmaceutical products. HPLC is the benchmark analytical method in the pharmaceutical industry due to its ability to ...
Hillel Brandes
Reporter EU Volume 30
Keywords: Anti-depressants, Chromatography, Diffusion, High performance liquid chromatography, Mass spectrometry, PAGE, Pharmaceutical, Reductions, Separation

Selective Extraction and Cleanup of Patulin Mycotoxin from Apple Juice Samples

Introduction Patulin (Figure 1) is a mycotoxin produced by a number of fungal species. The mold Penicillium Expansum, in particular, is known as the main source of patulin contamination[1]. Patulin o...
Olga Shimelis, K. G. Espenschied, and Jennifer Claus
Reporter US, Volume 32.1
Keywords: Adsorption, Chromatography, Gas chromatography, High performance liquid chromatography, Mass spectrometry, Phase transitions, Polymerization reactions, Sample preparations, Separation, Size-exclusion chromatography, Solid phase extractions

Serum Lipidomics Profiling | Ascentis Express Fused-Core C18 Column

The following was generated with the assistance of an outside source using Sigma-Aldrich products. Technical content was generated and provided by:
Reporter US Volume 29.5
Keywords: Analytical Chemistry, Chromatography, Diffusion, Gas chromatography, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Metabolites, Phase transitions, Separation

Related Content

Boosting HPLC Productivity: Nick the Hero [VIDEO]

Boosting HPLC Productivity Ascentis Express columns provide high-speed and high-efficiency separations that boost lab productivity. Learn about the features and benefits; become the "Nick the Hero" i...
Keywords: High performance liquid chromatography

Selection Guide for Ascentis® Express HPLC Columns

Ascentis Express C18 is the first choice for starting a new method. However, when a C18 doesn't give the desired separation or your sample contains compounds that are known to be difficult to retain ...
Keywords: High performance liquid chromatography, Separation, Supercritical fluid chromatography

Supelco HPLC Calculator for Isocratic or Gradient Method Transfer

Calculates conditions for transfer of an isocratic or gradient method from one HPLC column to another. Allows method scaling from microbore through preparative column range. Calculates time and solve...
Keywords: High performance liquid chromatography

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53501-U Ascentis® Express C18, 2.7 Micron Guard Cartridge, 2.7 μm particle size, L × I.D. 5 mm × 2.1 mm, pkg of 3 ea

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