• USA Home
  • 54805 - Disposable PD-10 Desalting Column

54805 Supelco

Disposable PD-10 Desalting Column



material   polypropylene (with polyethylene frit)
packaging   pkg of 30 ea
storage temp.   2-8°C


Frequently Asked Questions

Frequently Asked Questions are available for this Product.


Disposable PD-10 Desalting Column has been used:
• to concentrate samples of high peroxidase activity from a PGF-HRP (prostaglandin F2α-horse radish peroxidase) conjugate solution and to remove free PGF from this solution.
• for the separation of lanthanide-labeled proteins from unbound lanthanides by column chromatography.
• for repurification of protein by removal of lower molecular weight fragments after elution from Ni-NTA agarose affinity column.
• for separation of Eu-labeled protein from free Eu (Europium).

PD-10 desalting columns have been used to purify reduced Alemtuzumab (Campath, Berlex) humanized IgG1 rat monoclonal antibody from excess phospine, which is being developed for the treatment of chronic lymphocytic leukemia, autoimmune disease and for the prevention of transplant rejection.

General description

The PD-10 desalting columns contain Sephadex® G-25 for gel filtration (size exclusion) of biomolecules. Globular compounds pass quickly through the column. Compounds, such as salts, pass through the column slowly. The column actually separates two classes of compounds by their molecular weight, thus desalting the protein of interest.

Legal Information

Sephadex is a registered trademark of GE Healthcare

Price and Availability

Tools for Large Molecule BioAnalysis
Safety & Documentation

Safety Information

GHS07  GHS07
Signal word 
Hazard statements 
Precautionary statements 
NONH for all modes of transport


Certificate of Analysis

Protocols & Articles


Selection Guide for Polymeric Resins in Chromatography & Purification

Sigma-Aldrich carries a vast array of resins, from leading manufacturers; such as, Dow Chemical, Rohm & Haas, Mitsubishi, and Lanxess. Our product line includes adsorbent, ion-exchange (anion / catio...
Keywords: Adsorption, Antibiotics, Detergents, Fermentation, Filtration, Hormones, Ion Exchange, Pharmaceutical, Purification, Separation

Peer-Reviewed Papers


Set your institution to view full text papers.
Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?