|Related Categories||Analytical/Chromatography, Flash Chromatography, HiTrap & PD-10 Cartridges, Pharmacia, Prepacked LPLC Columns,|
|packaging||pkg of 5 × 5 mL|
|compatibility||mode of use strong cation exchange chromatography|
HiTrap® SP and HiTrap® Q columns are prepacked, ready-to-use columns, suitable for syringe or peristaltic pump use (adapters included).
Recommended flow rate: 1 mL/min
Maximum back pressure: ~40 psi (3 bar)
Chemical stability: All commonly used aqueous buffers, 1 M sodium hydroxide, 8 M urea, 6 M guanidine, 70% ethanol.
HiTrap® Q: strong anion exhange Sepharose® media for use at pH above the pI of the biomolecule (molecule will carry a negative net charge). Choose Q if the sample components are most stable above their pI.
HiTrap® SP: strong cation exhange Sepharose® media for use at pH below the pI of the biomolecule (molecule will carry a positive net charge). Choose SP if the sample components are most stable below their pI.
HiTrap is a registered trademark of GE Healthcare
Sepharose is a registered trademark of GE Healthcare
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