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55416-U Supelco

PHOS-Select Gallium Silica Spin Column Kit

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Description

Application

The PHOS-Select Gallium Silica Spin Column is a convenient tool for phosphopeptide enrichment from crude samples following tryptic digestion, using immobilized metal affinity chromatography (IMAC).

Features and Benefits

• Low non-specific binding - high data confidence
• No bias in phosphorylation states - accurate representation of phosphorylation types present for sample comparison
• Fast sample processing - reduces time to data generation from sample collection

General description

Chelated Gallium (III) exhibits high affinity and selectivity for phosphopeptides. The capture matrix is a novel Ga3+ chelate silica based on a proprietary nitriloacetic acid (NTA) analog. The matrix is packed into spin columns for easy, microscale affinity capture of phosphopeptides. The enriched sample helps to overcome the limitations of mass spectrometry of phosphopeptides.
The pre-packed spin column contains 50 μl (25 mg) of phosphopeptide capture matrix; each column can purify up to 25 nmoles of phosphopeptides.
Kit Contents:
PHOS-Select™ Gallium Silica Spin Columns 24 each
Collection tubes 100 each

Legal Information

PHOS-Select is a trademark of Sigma-Aldrich Co. LLC

Price and Availability

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

Protocols & Articles
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