|Related Categories||Analytical column, 3 micron, Analytical/Chromatography, Ascentis HPLC Columns, Ascentis RP-Amide, HPLC,|
|extent of labeling||19.5% Carbon loading|
|limit||≤70 °C temp. range|
|L × I.D.||2 cm × 4.6 mm|
|surface coverage||2.7 μmol/m2|
|impurities||<5 ppm metals|
|matrix||silica gel high purity, spherical|
|matrix active group||amido embedded reversed-phase|
|particle size||3 μm|
|pore size||100 Å|
|surface area||450 m2/g|
|operating pH range||2 - 8|
|suitability||suitable for L60 per USP|
• Excellent retention and peak shape for polar compounds
• 100% aqueous compatibility
• Ultra low bleed, LC-MS compatible
• Unique selectivity
The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide.
Ascentis RP-Amide is a new generation ultra low bleed, embedded polar group (EPG) phase that provides orthogonal selectivity and increased resolution for HPLC and LC-MS analysis of polar compounds. The Ascentis RP-Amide is the first choice in embedded polar group HPLC phases.
Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
Calculates conditions for transfer of an isocratic or gradient method from one HPLC column to another. Allows method scaling from microbore through preparative column range. Calculates time and solve...
Keywords: High performance liquid chromatography
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