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567526-U Supelco

Discovery® HS F5 HPLC Column

10 μm particle size, L × I.D. 25 cm × 10 mm

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Properties

Related Categories Analytical/Chromatography, Discovery HPLC Columns, Discovery HS F5 Prep Columns, HPLC, HPLC Columns, by Brand for Small Molecules,
feature   endcapped
extent of labeling   12% Carbon loading
parameter   ≤70 °C temp. range
L × I.D.   25 cm × 10 mm
surface coverage   4 μmol/m2
impurities   <10 ppm metals
matrix   silica gel, high purity, spherical particle platform
matrix active group   PFP (pentafluorophenyl) phase
particle size   10 μm
pore size   120 Å
surface area   300 m2/g
operating pH range   2 - 8
suitability   suitable for L43 per USP
mode of chromatography   Hydrophilic interaction (HILIC)
  Reversed phase

Description

Features and Benefits

• Unique selectivity
• Similar retention to C18 (sometimes requires stronger mobile phase)
• Excellent peak shape
• Stable, low-bleed LC-MS separations
• Scalable separations from 3 to 10μm particle sizes

General description

Guidelines for transferring a C18 method to Discovery® HS F5:
Generally, bases are retained longer on the HS F5 than on a C18. Increasing the organic content of a C18 separation 5 to 10 percent will generally provide similar retention on an HS F5. Results with other compounds are highly variable. However, it is generally true that solutes with log Po/w values less than 2.5 will be retained longer on HS F5 compared to a C18. The degree of difference is highly solute dependent.

The Discovery® HS F5 bonded phase provides reversed-phase separations that are distinctly different from C18 columns. However, compounds will generally elute within the same retention time window, making most C18 methods easily transferable.

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Legal Information

Discovery is a registered trademark of Sigma-Aldrich Co. LLC

Price and Availability


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Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

Protocols & Articles

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