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581615-U Supelco

Ascentis® Phenyl HPLC Column

5 μm particle size, L × I.D. 5 cm × 4.6 mm

  •  eCl@ss 32110501

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Properties

Related Categories Analytical column, 5 micron, Analytical/Chromatography, Ascentis HPLC Columns, Ascentis Phenyl, HPLC,
feature   endcapped
extent of labeling   19% Carbon loading
limit   ≤70 °C temp. range
application(s)   HPLC: suitable
L × I.D.   5 cm × 4.6 mm
surface coverage   5.2 μmol/m2
impurities   <5 ppm metals
matrix   silica gel high purity, spherical
matrix active group   phenyl phase
particle size   5 μm
pore size   100 Å
surface area   450 m2/g
operating pH range   2 - 8
suitability   suitable for L11 per USP
mode of chromatography   Reversed phase

Description

Features and Benefits

• 100% Aqueous Compatible
• ANP/HILIC and reversed-phase
• Low UV/MS bleed for gradient applications
• Alternate selectivity

General description

The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide.

The Ascentis Phenyl provides superior separations in reversed-phase mode including 100% aqueous conditions. It may also be used in HILIC/ANP (aqueous normal phase) mode and shows low UV/MS bleed for gradient applications.

Legal Information

Ascentis is a registered trademark of Sigma-Aldrich Co. LLC

Price and Availability

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

Protocols & Articles

Articles

HPLC Separation of Fluorinated Pharmaceutical Compounds on Supelco Ascentis Columns

Fluorinated compounds possess distinct characteristics that confer desirable physico-chemical properties compared to their non-fl uorinated analogs. Their abundance in pharmaceuticals, chemicals, con...
Jacinth A. M. McKenzie, Wayne K. Way
Reporter EU Volume 26
Keywords: Absorption, Applications, Biochemistry, Cancer, Cosmetics, Degradations, Environmental, Fluorinations, Forensic, Halogenations, Help, High performance liquid chromatography, Mass spectrometry, Metabolites, Methods, Oxidations, Pharmaceutical, Separation, Substitutions

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Supelco HPLC Calculator for Isocratic or Gradient Method Transfer

Calculates conditions for transfer of an isocratic or gradient method from one HPLC column to another. Allows method scaling from microbore through preparative column range. Calculates time and solve...
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