Discovery® BIO Wide Pore C18 HPLC Column
5 μm particle size, L × I.D. 15 cm × 0.18 mm
DOWNLOAD MSDS (PDF)Properties
| Related Categories | 5 micron, Analytical/Chromatography, C18, C18 HPLC Columns, Capillary & Microbore Dimensions, |
| feature | endcapped |
| extent of labeling | 9.2% Carbon loading |
| L × I.D. | 15 cm × 0.18 mm |
| surface coverage | 3.6 μmol/m2 |
| matrix | silica particle platform |
| matrix active group | octadecyl phase |
| particle size | 5 μm |
Description
General description
Peptide maps generated by RP-HPLC provide valuable information about protein structure, stability, and purity. To be effective, the RP-HPLC column must be able to resolve a high percentage of the peptides in the sample. The more peptides, the better the information. Discovery BIO Wide Pore C18 gives unsurpassed RP-HPLC resolution of peptide maps from enzymatic digests. The improvements in silica and bonded-phase chemistry we have incorporated into the Discovery BIO Wide Pore line improve resolution by increasing efficiency and reducing the peak tailing. An added benefit to this is the ability to analyze peptides without TFA in the mobile phase, thereby increasing the LC-MS signal.
Legal Information
Discovery is a registered trademark of Sigma-Aldrich Co. LLC
Price and Availability
NEW! Ascentis® Express 5 µm
HPLC Columns
Ascentis Express columns, based on 5 µm Fused-Core® particles, offer better performance, faster and more efficient separations, compared to columns based on 3 µm and 5 µm totally porous particles, with no backpressure concerns!Technical Service:
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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