|Related Categories||5 micron, Analytical/Chromatography, C18, C18 HPLC Columns, Capillary & Microbore Dimensions,|
|extent of labeling||9.2% Carbon loading|
|L × I.D.||15 cm × 0.18 mm|
|surface coverage||3.6 μmol/m2|
|matrix||silica particle platform|
|matrix active group||octadecyl phase|
|particle size||5 μm|
|pore size||300 Å|
|surface area||100 m2/g|
|suitability||suitable for L1 per USP|
Peptide maps generated by RP-HPLC provide valuable information about protein structure, stability, and purity. To be effective, the RP-HPLC column must be able to resolve a high percentage of the peptides in the sample. The more peptides, the better the information. Discovery BIO Wide Pore C18 gives unsurpassed RP-HPLC resolution of peptide maps from enzymatic digests. The improvements in silica and bonded-phase chemistry we have incorporated into the Discovery BIO Wide Pore line improve resolution by increasing efficiency and reducing the peak tailing. An added benefit to this is the ability to analyze peptides without TFA in the mobile phase, thereby increasing the LC-MS signal.
Discovery is a registered trademark of Sigma-Aldrich Co. LLC
Calculates conditions for transfer of an isocratic or gradient method from one HPLC column to another. Allows method scaling from microbore through preparative column range. Calculates time and solve...
Keywords: High performance liquid chromatography
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