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807656 Supelco

TSKgel® Phenyl-5PW HPLC Column

phase phenyl, L × I.D. 15 cm × 21.5 mm, 13 μm particle size, stainless steel hardware

Synonym: TSKgel® Hydrophobic Interaction Chromatography (HIC) Column

  •  eCl@ss 32110501

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Properties

Related Categories Analytical/Chromatography, HPLC, HPLC & FPLC Columns for Biopolymer Separations, Hydrophobic Interaction (HIC) Columns, TSKgel HPLC Columns, TOYOPEARL Bulk Media & Accessories,
material   stainless steel hardware
mfr. no.   Tosoh Phenyl-5PW, 07656
parameter   ≤4 M salt concentration
  ≤50% organic solvent
  10-50 °C temperature
  20 bar max. pressure
  8 mL/min flow rate
application(s)   HPLC: suitable
L × I.D.   15 cm × 21.5 mm
matrix   polymer particle platform
matrix active group   phenyl phase
particle size   13 μm
pore size   1000 Å
operating pH   2 - 12
mode of chromatography   Hydrophobic interaction (HIC)

Description

General description

Both hydrophobic interaction chromatography (HIC) and reversed-phase liquid chromatography separate on the basis of protein hydrophobicity and allow selective binding and desorption of proteins. However, HIC operates at significantly lower binding energy and uses aqueous mobile phases. These characteristics provide a gentle technique that is less likely to disturb protein conformation. As a result, HIC generally provides better activity recovery.

TSKgel BioAssist Phenyl columns contain the same particles as Phenyl-5PW columns, but are packed in PEEK column hardware rather than stainless steel. BioAsist Phenyl (Phenyl-5PW), TSKgel Ether-5PW and Butyl-NPR resin-based columns provide a range of hydrophobicities for chromatographic optimization. Ammonium sulfate concentration can be minimized by using a more hydrophobic packing, such as BioAssist Phenyl. We recommend an Ether-5PW column for purifying very hydrophobic proteins. TSKgel BioAssist Phenyl and TSKgel Ether-5PW packings are based on TSKgel G5000PW resin - 10 μm particles with 1000 Å pores. TSKgel Butyl-NPR columns, which are less hydrophobic than Ether-5PW, is prepared from 2.5 μm nonporous particles, allowing for rapid analyses and semi-micropreparative applications. All three column types can be cleaned with 0.2 M NaOH. Scale-up can be performed by using corresponding Toyopearl bulk resins.

The most hydrophobic among the three TSKgel HIC columns, TSKgel Phenyl-5PW is an excellent choice to screen for the selectivity, retention and recovery of most biomolecules. Featuring a 1000Å pore size in a polymethacrylate base material, the Phenyl-5PW column is stable in either acid or caustic cleaning regimens and provides excellent access to larger molecules with low diffusion coefficients. Stainless steel is the standard column format for TSKgel Phenyl-5PW. Glass and PEEK column housings are available for samples that may adsorb to stainless steel. The alternative column formats are also suitable for general applications.

Physical form

Shipped in water.

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Legal Information

TSKgel is a registered trademark of Tosoh Corporation

Price and Availability


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Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

HPLC Analysis of Large RNAs on TSKgel® Phenyl-5PW

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Large RNAs on TSKgel® Phenyl-5PW
Keywords: Chromatography, High performance liquid chromatography

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816095 TSKgel® Phenyl-5PW Guardgel Kit, phase phenyl, L × I.D. 3.5 cm × 10 mm, 20 μm particle size

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