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808643 Supelco

TSKgel® Ether-5PW Guardgel Kit

phase ether, L × I.D. 2.5 cm × 6 mm, 20 μm particle size

Synonym: TSKgel® Ether-5PW HPLC Column

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Properties

Related Categories Analytical/Chromatography, HPLC, HPLC & FPLC Columns for Biopolymer Separations, Hydrophobic Interaction (HIC) Columns, TSKgel Ether-5PW Column (1000 Å pore size),
mfr. no.   Tosoh Ether-5PW, 08643
limit   ≤4 M salt concentration
  ≤50% organic solvent
  10-50 °C temperature
L × I.D.   2.5 cm × 6 mm
matrix   polymer particle platform
matrix active group   ether phase
particle size   20 μm
operating pH   2 - 12
mode of chromatography   Hydrophobic interaction (HIC)

Description

General description

Both hydrophobic interaction chromatography (HIC) and reversed-phase liquid chromatography separate on the basis of protein hydrophobicity and allow selective binding and desorption of proteins. However, HIC operates at significantly lower binding energy and uses aqueous mobile phases. These characteristics provide a gentle technique that is less likely to disturb protein conformation. As a result, HIC generally provides better activity recovery.
Three resin-based TSKgel HIC columns provide a range of hydrophobicities for chromatographic optimization. TSKgel Phenyl-5PW columns are most hydrophobic, followed by Ether-5PW, while the surface of Butyl-NPR columns is most polar or least hydrophobic. Ammonium sulfate concentration can be minimized by using a more hydrophobic packing such as Phenyl-5PW. We recommend the more polar Ether-5PW column for purifying more hydrophobic proteins. Ether-5PW and Phenyl-5PW packings are based on TSKgel G5000PW resin - 10 micron particles with 1000  pores. Butyl-NPR packing is prepared from 2.5 micron nonporous particles, allowing for rapid analyses and semi-micropreparative applications. All three column types can be cleaned with 0.2 M NaOH. Scale-up can be performed by using corresponding Toyopearl bulk resins.

TSKgel Guardgel kits contain:
• 5 mL Guardgel packing material
• Column holder, consisting of external reducing union, cap, frit, 2 ferrules
• Column, 25 mm x 9.5 mm OD x 6 mm ID, 2 μm frit at 10 mm depth, 1/16"" exit
• 2 Replacement frits
• Capillary tubing, 5 cm x 1/16"" OD x 0.01"" ID
• Nuts and ferrules, 10-32 threads for 1/16"" tubing, 2 each
• All hardware is 316 stainless steel

Dimensions for Guardgel kits for 21.5 mm ID columns:
• Column, 35 mm x 12.75 mm OD x 10 mm ID, 10 μm frit at 20mm depth, 1/16"
• Capillary tubing, 5 cm x 1/16"" OD x 0.02"" ID

Legal Information

TSKgel is a registered trademark of Tosoh Corporation

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Certificate of Analysis

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808641 TSKgel® Ether-5PW HPLC Column, phase ether, L × I.D. 7.5 cm × 7.5 mm, 10 μm particle size

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