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813125 Supelco

TSKgel® Boronate-5PW HPLC Guardgel Kit

phase m-aminophenyl boronate, L × I.D. 2.5 cm × 6 mm, 20 μm particle size

Synonym: TSKgel® Boronate-5PW HPLC Guardgel Kit

  •  eCl@ss 32110501

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Properties

Related Categories Analytical/Chromatography, HPLC, HPLC & FPLC Columns for Biopolymer Separations, TSKgel Affinity HPLC Columns, TSKgel HPLC Columns, TOYOPEARL Bulk Media & Accessories More...
mfr. no.   Tosoh Boronate-5PW, 13125
parameter   ≤20% organic solvent
  ≤3 M salt concentration
  ≥ 0 °C temperature
application(s)   HPLC: suitable
L × I.D.   2.5 cm × 6 mm
matrix   polymer particle platform
matrix active group   m-aminophenyl boronate phase
particle size   20 μm
pore size   1000 Å
operating pH   2 - 9
capacity   40 μmol/mL, resin adsorption capacity (sorbitol)
compatibility   mode of use affinity HPLC
mode of chromatography   Affinity
storage temp.   2-8°C

Description

Components

TSKgel Guardgel kits contain:
• 5 mL Guardgel packing material
• Column holder, consisting of external reducing union, cap, frit, 2 ferrules
• Column, 25 mm x 9.5 mm OD x 6 mm ID, 2 μm frit at 10 mm depth, 1/16"
• 2 Replacement frits
• Capillary tubing, 5 cm x 1/16" OD x 0.01"
• Nuts and ferrules, 10-32 threads for 1/16" tubing, 2 each
• All hardware is 316 stainless steel

Dimensions for Guardgel kits for 21.5 mm ID columns:
• Column, 35 mm x 12.75 mm OD x 10 mm ID, 10 μm frit at 20mm depth, 1/16"
• Capillary tubing, 5 cm x 1/16" OD x 0.02" ID

General description

Guardgel Kit for 7.5mmID TSKgel Boronate-5PW columns

High efficiency, resin-based TSKgel affinity columns (10 μm particles) separate or purify many enzymes and other proteins.

Coupling of m-aminophenyl boronate to TSKgel 5PW polymeric support results in a ligand capable of forming a tetrahedral boronate anion under alkaline pH conditions. This anionic structure can bind with 1,2 cis-diol groups such as those found in carbohydrates, carbohydrate containing compounds and catecholamines. Interaction between the boronate anion and the 1,2 cis-diol groups is enhanced in the presence of Mg2+ ions and is inhibited by amine-containing buffers. Adsorption onto the TSKgel Boronate-5PW matrix takes place in basic buffers such as HEPES and morpholine while desorption takes place in carbohydrate or amine-containing mobile phase like sorbitol or Tris.

Applications include: nucleic acids, catecholamines, nucleosides and nucleotides.

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Legal Information

TSKgel is a registered trademark of Tosoh Corporation

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Safety & Documentation

Safety Information

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Documents

Certificate of Analysis

Protocols & Articles

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