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814456 Supelco

TSKgel® Tresyl-5PW HPLC Column

phase tresyl, L × I.D. 7.5 cm × 7.5 mm, 10 μm particle size

Synonym: TSKgel® Affinity HPLC Column and Media

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Properties

Related Categories Analytical/Chromatography, HPLC, HPLC & FPLC Columns for Biopolymer Separations, TSKgel Affinity HPLC Columns, TSKgel HPLC Columns, TOYOPEARL Bulk Media & Accessories More...
material   stainless steel hardware
mfr. no.   Tosoh Tresyl-5PW, 14456
parameter   ≤20% organic solvent
  ≤3 M salt concentration
  >0 °C temperature
  1.2 mL/min flow rate
  10 bar max. pressure
L × I.D.   7.5 cm × 7.5 mm
matrix   polymer particle platform
matrix active group   tresyl phase
particle size   10 μm
operating pH   2 - 12
capacity   >60 mg/g, dry resin coupling capacity (soybean trypsin inhibitor)
mode of chromatography   Affinity

Description

General description

High efficiency, resin-based TSKgel affinity columns (10 μm particles) separate or purify many enzymes and other proteins.

Unlike other TSKgel affinity columns, TSKgel Tresyl-5PW, which is derivatized with the 2,2,2-trifluroethanesulfonyl ligand, requires activation with a user-selected ligand containing amino, thiol, phenol, or imidazole groups. The resulting structure is literally a "custom" affinity ligand with excellent pH stability and minimal ligand loss due to leaching. TSKgel Tresyl-5PW readily reacts with amino or thiol groups to form stable covalent alkylamines or thioethers.

Applications include: glycoproteins, antigens, etc.

Legal Information

TSKgel is a registered trademark of Tosoh Corporation

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Documents

Certificate of Analysis

Protocols & Articles

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