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816260 Supelco

TSKgel® Phenyl-5PW RP HPLC Column

phase phenyl, L × I.D. 15 cm × 21.5 mm, 13 μm particle size

Synonym: TSKgel® Reversed Phase HPLC Column (Polymer-based)

  •  eCl@ss 32110501

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Properties

Related Categories Analytical/Chromatography, HPLC, HPLC & FPLC Columns for Biopolymer Separations, TSKgel HPLC Columns, TOYOPEARL Bulk Media & Accessories, TSKgel Phenyl-5PW RP Columns (1000 Å pores),
material   stainless steel hardware
mfr. no.   Tosoh Phenyl-5PW, 16260
extent of labeling   1 meq/mL ligand density (estimate)
parameter   salt concentration
  0-100% organic solvent
  30 bar max. pressure
  4-60 °C temperature
  8 mL/min flow rate
application(s)   HPLC: suitable
L × I.D.   15 cm × 21.5 mm
matrix   polymer particle platform
matrix active group   phenyl phase
particle size   13 μm
pore size   1000 Å
operating pH   2 - 12
mode of chromatography   Reversed phase

Description

General description

Polymer-based particles for reversed-phase (RP) HPLC generally have two benefits over silica-based particles: They are more tolerant of high and low pH operating conditions and they do not have the silanol interactions that cause peak tailing of some basic analytes. Also, some silicas have an intrinsic metal content that may cause chelation problems with sensitive biomolecules, leading to poor recovery. Polymeric TSKgel columns come in C18 and phenyl functional groups and a range of particle porosities. Fast kinetics and quantitative protein recovery at submicrogram loading are characteristic of nonporous resin-based TSKgel Octadecyl-NPR columns.
• Octadecyl-2PW particles have 125Å pores
• Octadecyl-4PW particles have 500Å pores
• Phenyl-5PW RP particles have 1000Å pores
• Octadecyl-NPR particles are nonporous

TSKgel Phenyl-5PW RP offers similiar selectivity to its hydrophobic interation counterpart TSKgel Phenyl-5PW, although a higher degree of cross-linking allows for increased stability in aqueous/organic mobile phase gradients. The highly cross-linked polymethacrylate base material provides an advantage over silica when high pH buffer systems are needed. Additionally, TSKgel Phenyl-5PW RP can withstand rigorous cleaning protocols using either acid or base. The 1000Å pore size accommodates globular protein samples up to 1,000,000 Da.

Physical form

Shipped in 30% methanol - 70% water.

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Legal Information

TSKgel is a registered trademark of Tosoh Corporation

Price and Availability


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Safety & Documentation

Safety Information

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Documents

Certificate of Analysis

Protocols & Articles

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